Influenza virus mRNAs encode determinants for nuclear export via the cellular TREX-2 complex

Prasanna Bhat, Vasilisa Aksenova, Matthew Gazzara, Emily A. Rex, Sadaf Aslam, Christina Haddad, Shengyan Gao, Matthew Esparza, Tolga Cagatay, Kimberly Batten, Sara S. El Zahed, Alexei Arnaoutov, Hualin Zhong, Jerry W. Shay, Blanton S. Tolbert, Mary Dasso, Kristen W. Lynch, Adolfo García-Sastre and Beatriz M. A. Fontoura ()
Additional contact information
Prasanna Bhat: University of Texas Southwestern Medical Center
Vasilisa Aksenova: National Institutes of Health
Matthew Gazzara: University of Pennsylvania
Emily A. Rex: University of Texas Southwestern Medical Center
Sadaf Aslam: Icahn School of Medicine at Mount Sinai
Christina Haddad: Case Western Reserve University
Shengyan Gao: University of Texas Southwestern Medical Center
Matthew Esparza: University of Texas Southwestern Medical Center
Tolga Cagatay: University of Texas Southwestern Medical Center
Kimberly Batten: University of Texas Southwestern Medical Center
Sara S. El Zahed: Icahn School of Medicine at Mount Sinai
Alexei Arnaoutov: National Institutes of Health
Hualin Zhong: Hunter College
Jerry W. Shay: University of Texas Southwestern Medical Center
Blanton S. Tolbert: Case Western Reserve University
Mary Dasso: National Institutes of Health
Kristen W. Lynch: University of Pennsylvania
Adolfo García-Sastre: Icahn School of Medicine at Mount Sinai
Beatriz M. A. Fontoura: University of Texas Southwestern Medical Center

Nature Communications, 2023, vol. 14, issue 1, 1-14

Abstract: Abstract Nuclear export of influenza A virus (IAV) mRNAs occurs through the nuclear pore complex (NPC). Using the Auxin-Induced Degron (AID) system to rapidly degrade proteins, we show that among the nucleoporins localized at the nucleoplasmic side of the NPC, TPR is the key nucleoporin required for nuclear export of influenza virus mRNAs. TPR recruits the TRanscription and EXport complex (TREX)−2 to the NPC for exporting a subset of cellular mRNAs. By degrading components of the TREX-2 complex (GANP, Germinal-center Associated Nuclear Protein; PCID2, PCI domain containing 2), we show that influenza mRNAs require the TREX-2 complex for nuclear export and replication. Furthermore, we found that cellular mRNAs whose export is dependent on GANP have a small number of exons, a high mean exon length, long 3’ UTR, and low GC content. Some of these features are shared by influenza virus mRNAs. Additionally, we identified a 45 nucleotide RNA signal from influenza virus HA mRNA that is sufficient to mediate GANP-dependent mRNA export. Thus, we report a role for the TREX-2 complex in nuclear export of influenza mRNAs and identified RNA determinants associated with the TREX-2-dependent mRNA export.

Date: 2023
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DOI: 10.1038/s41467-023-37911-0

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