Dynamic changes of the Prf/Pto tomato resistance complex following effector recognition

Arsheed H. Sheikh, Iosif Zacharia, Alonso J. Pardal, Ana Dominguez-Ferreras, Daniela J. Sueldo, Jung-Gun Kim, Alexi Balmuth, Jose R. Gutierrez, Brendon F. Conlan, Najeeb Ullah, Olivia M. Nippe, Anil M. Girija, Chih-Hang Wu, Guido Sessa, Alexandra M. E. Jones, Murray R. Grant, Miriam L. Gifford, Mary Beth Mudgett, John P. Rathjen and Vardis Ntoukakis ()
Additional contact information
Arsheed H. Sheikh: University of Warwick
Iosif Zacharia: University of Warwick
Alonso J. Pardal: University of Warwick
Ana Dominguez-Ferreras: University of Warwick
Daniela J. Sueldo: University of Warwick
Jung-Gun Kim: Stanford University
Alexi Balmuth: J.R. Simplot Company
Jose R. Gutierrez: Norwich Research Park
Brendon F. Conlan: The Australian National University
Najeeb Ullah: University of Warwick
Olivia M. Nippe: University of Warwick
Anil M. Girija: Tel-Aviv University
Chih-Hang Wu: Norwich Research Park
Guido Sessa: Tel-Aviv University
Alexandra M. E. Jones: University of Warwick
Murray R. Grant: University of Warwick
Miriam L. Gifford: University of Warwick
Mary Beth Mudgett: Stanford University
John P. Rathjen: The Australian National University
Vardis Ntoukakis: University of Warwick

Nature Communications, 2023, vol. 14, issue 1, 1-11

Abstract: Abstract In both plants and animals, nucleotide-binding leucine-rich repeat (NLR) immune receptors play critical roles in pathogen recognition and activation of innate immunity. In plants, NLRs recognise pathogen-derived effector proteins and initiate effector-triggered immunity (ETI). However, the molecular mechanisms that link NLR-mediated effector recognition and downstream signalling are not fully understood. By exploiting the well-characterised tomato Prf/Pto NLR resistance complex, we identified the 14-3-3 proteins TFT1 and TFT3 as interacting partners of both the NLR complex and the protein kinase MAPKKKα. Moreover, we identified the helper NRC proteins (NLR-required for cell death) as integral components of the Prf /Pto NLR recognition complex. Notably our studies revealed that TFTs and NRCs interact with distinct modules of the NLR complex and, following effector recognition, dissociate facilitating downstream signalling. Thus, our data provide a mechanistic link between activation of immune receptors and initiation of downstream signalling cascades.

Date: 2023
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DOI: 10.1038/s41467-023-38103-6

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