Structures of Tetrahymena thermophila respiratory megacomplexes on the tubular mitochondrial cristae

Fangzhu Han, Yiqi Hu, Mengchen Wu, Zhaoxiang He, Hongtao Tian and Long Zhou ()
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Fangzhu Han: Zhejiang University School of Medicine
Yiqi Hu: Zhejiang University School of Medicine
Mengchen Wu: Zhejiang University School of Medicine
Zhaoxiang He: Zhejiang University School of Medicine
Hongtao Tian: Zhejiang University School of Medicine
Long Zhou: Zhejiang University School of Medicine

Nature Communications, 2023, vol. 14, issue 1, 1-13

Abstract: Abstract Tetrahymena thermophila, a classic ciliate model organism, has been shown to possess tubular mitochondrial cristae and highly divergent electron transport chain involving four transmembrane protein complexes (I–IV). Here we report cryo-EM structures of its ~8 MDa megacomplex IV2 + (I + III2 + II)2, as well as a ~ 10.6 MDa megacomplex (IV2 + I + III2 + II)2 at lower resolution. In megacomplex IV2 + (I + III2 + II)2, each CIV2 protomer associates one copy of supercomplex I + III2 and one copy of CII, forming a half ring-shaped architecture that adapts to the membrane curvature of mitochondrial cristae. Megacomplex (IV2 + I + III2 + II)2 defines the relative position between neighbouring half rings and maintains the proximity between CIV2 and CIII2 cytochrome c binding sites. Our findings expand the current understanding of divergence in eukaryotic electron transport chain organization and how it is related to mitochondrial morphology.

Date: 2023
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DOI: 10.1038/s41467-023-38158-5

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