A link between STK signalling and capsular polysaccharide synthesis in Streptococcus suis

Jinsheng Tang, Mengru Guo, Min Chen, Bin Xu, Tingting Ran, Weiwu Wang, Zhe Ma, Huixing Lin and Hongjie Fan ()
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Jinsheng Tang: Nanjing Agricultural University
Mengru Guo: Nanjing Agricultural University
Min Chen: Nanjing Agricultural University
Bin Xu: Jiangsu Academy of Agricultural Sciences
Tingting Ran: Nanjing Agricultural University
Weiwu Wang: Nanjing Agricultural University
Zhe Ma: Nanjing Agricultural University
Huixing Lin: Nanjing Agricultural University
Hongjie Fan: Nanjing Agricultural University

Nature Communications, 2023, vol. 14, issue 1, 1-22

Abstract: Abstract Synthesis of capsular polysaccharide (CPS), an important virulence factor of pathogenic bacteria, is modulated by the CpsBCD phosphoregulatory system in Streptococcus. Serine/threonine kinases (STKs, e.g. Stk1) can also regulate CPS synthesis, but the underlying mechanisms are unclear. Here, we identify a protein (CcpS) that is phosphorylated by Stk1 and modulates the activity of phosphatase CpsB in Streptococcus suis, thus linking Stk1 to CPS synthesis. The crystal structure of CcpS shows an intrinsically disordered region at its N-terminus, including two threonine residues that are phosphorylated by Stk1. The activity of phosphatase CpsB is inhibited when bound to non-phosphorylated CcpS. Thus, CcpS modulates the activity of phosphatase CpsB thereby altering CpsD phosphorylation, which in turn modulates the expression of the Wzx-Wzy pathway and thus CPS production.

Date: 2023
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DOI: 10.1038/s41467-023-38210-4

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