Cryogenic electron ptychographic single particle analysis with wide bandwidth information transfer

Xudong Pei, Liqi Zhou, Chen Huang, Mark Boyce, Judy S. Kim, Emanuela Liberti, Yiming Hu, Takeo Sasaki, Peter D. Nellist, Peijun Zhang, David I. Stuart, Angus I. Kirkland () and Peng Wang ()
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Xudong Pei: Nanjing University
Liqi Zhou: Nanjing University
Chen Huang: Harwell Science and Innovation Campus
Mark Boyce: University of Oxford
Judy S. Kim: Harwell Science and Innovation Campus
Emanuela Liberti: Harwell Science and Innovation Campus
Yiming Hu: Nanjing University
Takeo Sasaki: JEOL Ltd
Peter D. Nellist: University of Oxford
Peijun Zhang: University of Oxford
David I. Stuart: University of Oxford
Angus I. Kirkland: Harwell Science and Innovation Campus
Peng Wang: Nanjing University

Nature Communications, 2023, vol. 14, issue 1, 1-10

Abstract: Abstract Advances in cryogenic transmission electron microscopy have revolutionised the determination of many macromolecular structures at atomic or near-atomic resolution. This method is based on conventional defocused phase contrast imaging. However, it has limitations of weaker contrast for small biological molecules embedded in vitreous ice, in comparison with cryo-ptychography, which shows increased contrast. Here we report a single-particle analysis based on the use of ptychographic reconstruction data, demonstrating that three dimensional reconstructions with a wide information transfer bandwidth can be recovered by Fourier domain synthesis. Our work suggests future applications in otherwise challenging single particle analyses, including small macromolecules and heterogeneous or flexible particles. In addition structure determination in situ within cells without the requirement for protein purification and expression may be possible.

Date: 2023
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DOI: 10.1038/s41467-023-38268-0

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