Prenylcysteine oxidase 1 like protein is required for neutrophil bactericidal activities

Anastasiia Petenkova, Shelby A. Auger, Jeffrey Lamb, Daisy Quellier, Cody Carter, On Tak To, Jelena Milosevic, Rana Barghout, Abirami Kugadas, Xiaoxiao Lu, Jennifer Geddes-McAlister, Raina Fichorova, David B. Sykes, Mark D. Distefano and Mihaela Gadjeva ()
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Anastasiia Petenkova: Harvard Medical School
Shelby A. Auger: University of Minnesota
Jeffrey Lamb: Harvard Medical School
Daisy Quellier: Harvard Medical School
Cody Carter: Harvard Medical School
On Tak To: Harvard Medical School
Jelena Milosevic: Massachusetts General Hospital
Rana Barghout: Massachusetts General Hospital
Abirami Kugadas: Harvard Medical School
Xiaoxiao Lu: Harvard Medical School
Jennifer Geddes-McAlister: University of Guelph
Raina Fichorova: Harvard Medical School
David B. Sykes: Massachusetts General Hospital
Mark D. Distefano: University of Minnesota
Mihaela Gadjeva: Harvard Medical School

Nature Communications, 2023, vol. 14, issue 1, 1-16

Abstract: Abstract The bactericidal function of neutrophils is dependent on a myriad of intrinsic and extrinsic stimuli. Using systems immunology approaches we identify microbiome- and infection-induced changes in neutrophils. We focus on investigating the Prenylcysteine oxidase 1 like (Pcyox1l) protein function. Murine and human Pcyox1l proteins share ninety four percent aminoacid homology revealing significant evolutionary conservation and implicating Pcyox1l in mediating important biological functions. Here we show that the loss of Pcyox1l protein results in significant reductions in the mevalonate pathway impacting autophagy and cellular viability under homeostatic conditions. Concurrently, Pcyox1l CRISPRed-out neutrophils exhibit deficient bactericidal properties. Pcyox1l knock-out mice demonstrate significant susceptibility to infection with the gram-negative pathogen Psuedomonas aeruginosa exemplified through increased neutrophil infiltrates, hemorrhaging, and reduced bactericidal functionality. Cumulatively, we ascribe a function to Pcyox1l protein in modulation of the prenylation pathway and suggest connections beween metabolic responses and neutrophil functionality.

Date: 2023
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DOI: 10.1038/s41467-023-38447-z

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