A Yap-dependent mechanoregulatory program sustains cell migration for embryo axis assembly

Ana Sousa-Ortega, Javier Vázquez-Marín, Estefanía Sanabria-Reinoso, Jorge Corbacho, Rocío Polvillo, Alejandro Campoy-López, Lorena Buono, Felix Loosli, María Almuedo-Castillo () and Juan R. Martínez-Morales ()
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Ana Sousa-Ortega: Centro Andaluz de Biología del Desarrollo (CSIC/UPO/JA)
Javier Vázquez-Marín: Centro Andaluz de Biología del Desarrollo (CSIC/UPO/JA)
Estefanía Sanabria-Reinoso: Centro Andaluz de Biología del Desarrollo (CSIC/UPO/JA)
Jorge Corbacho: Centro Andaluz de Biología del Desarrollo (CSIC/UPO/JA)
Rocío Polvillo: Centro Andaluz de Biología del Desarrollo (CSIC/UPO/JA)
Alejandro Campoy-López: Centro Andaluz de Biología del Desarrollo (CSIC/UPO/JA)
Lorena Buono: Centro Andaluz de Biología del Desarrollo (CSIC/UPO/JA)
Felix Loosli: Institute of Biological and Chemical Systems, Biological Information Processing (IBCS-BIP), Karlsruhe Institute of Technology
María Almuedo-Castillo: Centro Andaluz de Biología del Desarrollo (CSIC/UPO/JA)
Juan R. Martínez-Morales: Centro Andaluz de Biología del Desarrollo (CSIC/UPO/JA)

Nature Communications, 2023, vol. 14, issue 1, 1-17

Abstract: Abstract The assembly of the embryo’s primary axis is a fundamental landmark for the establishment of the vertebrate body plan. Although the morphogenetic movements directing cell convergence towards the midline have been described extensively, little is known on how gastrulating cells interpret mechanical cues. Yap proteins are well-known transcriptional mechanotransducers, yet their role in gastrulation remains elusive. Here we show that the double knockout of yap and its paralog yap1b in medaka results in an axis assembly failure, due to reduced displacement and migratory persistence in mutant cells. Accordingly, we identified genes involved in cytoskeletal organization and cell-ECM adhesion as potentially direct Yap targets. Dynamic analysis of live sensors and downstream targets reveal that Yap is acting in migratory cells, promoting cortical actin and focal adhesions recruitment. Our results indicate that Yap coordinates a mechanoregulatory program to sustain intracellular tension and maintain the directed cell migration for embryo axis development.

Date: 2023
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DOI: 10.1038/s41467-023-38482-w

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