Near-lifespan longitudinal tracking of brain microvascular morphology, topology, and flow in male mice

Konrad W. Walek, Sabina Stefan, Jang-Hoon Lee, Pooja Puttigampala, Anna H. Kim, Seong Wook Park, Paul J. Marchand, Frederic Lesage, Tao Liu, Yu-Wen Alvin Huang, David A. Boas, Christopher Moore and Jonghwan Lee ()
Additional contact information
Konrad W. Walek: Brown University
Sabina Stefan: Brown University
Jang-Hoon Lee: Brown University
Pooja Puttigampala: Drexel University
Anna H. Kim: Brown University
Seong Wook Park: Brown University
Paul J. Marchand: École Polytechnique de Montréal
Frederic Lesage: École Polytechnique de Montréal
Tao Liu: Brown University School of Public Health
Yu-Wen Alvin Huang: Brown University
David A. Boas: Boston University
Christopher Moore: Brown University
Jonghwan Lee: Brown University

Nature Communications, 2023, vol. 14, issue 1, 1-13

Abstract: Abstract In age-related neurodegenerative diseases, pathology often develops slowly across the lifespan. As one example, in diseases such as Alzheimer’s, vascular decline is believed to onset decades ahead of symptomology. However, challenges inherent in current microscopic methods make longitudinal tracking of such vascular decline difficult. Here, we describe a suite of methods for measuring brain vascular dynamics and anatomy in mice for over seven months in the same field of view. This approach is enabled by advances in optical coherence tomography (OCT) and image processing algorithms including deep learning. These integrated methods enabled us to simultaneously monitor distinct vascular properties spanning morphology, topology, and function of the microvasculature across all scales: large pial vessels, penetrating cortical vessels, and capillaries. We have demonstrated this technical capability in wild-type and 3xTg male mice. The capability will allow comprehensive and longitudinal study of a broad range of progressive vascular diseases, and normal aging, in key model systems.

Date: 2023
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DOI: 10.1038/s41467-023-38609-z

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