Photoinhibiting via simultaneous photoabsorption and free-radical reaction for high-fidelity light-based bioprinting

Ning He, Xiaonan Wang, Liyang Shi, Jing Li, Lan Mo, Feng Chen (), Yuting Huang, Hairong Liu, Xiaolong Zhu, Wei Zhu, Yiqi Mao and Xiaoxiao Han ()
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Ning He: National Engineering Research Centre for High Efficiency Grinding, Hunan University
Xiaonan Wang: College of Biology, Hunan University
Liyang Shi: College of Biology, Hunan University
Jing Li: National Engineering Research Centre for High Efficiency Grinding, Hunan University
Lan Mo: Hunan Agricultural University
Feng Chen: National Engineering Research Centre for High Efficiency Grinding, Hunan University
Yuting Huang: Hunan University
Hairong Liu: Hunan University
Xiaolong Zhu: National Engineering Research Centre for High Efficiency Grinding, Hunan University
Wei Zhu: National Engineering Research Centre for High Efficiency Grinding, Hunan University
Yiqi Mao: National Engineering Research Centre for High Efficiency Grinding, Hunan University
Xiaoxiao Han: National Engineering Research Centre for High Efficiency Grinding, Hunan University

Nature Communications, 2023, vol. 14, issue 1, 1-15

Abstract: Abstract Light-based 3D bioprinting is now employed widely to fabricate geometrically complex constructs for various biomedical applications. However, the inherent light scattering defect creates significant challenges in patterning dilute hydrogels to form high-fidelity structures with fine-scale features. Herein, we introduce a photoinhibiting approach that can effectively suppress the light scattering effect via a mechanism of simultaneous photoabsorption and free-radical reaction. This biocompatible approach significantly improves the printing resolution (~1.2 - ~2.1 pixels depending on swelling) and shape fidelity (geometric error less than 5%), while minimising the costly trial-and-error procedures. The capability in patterning 3D complex constructs using different hydrogels is demonstrated by manufacturing various scaffolds featuring intricate multi-sized channels and thin-walled networks. Importantly, cellularised gyroid scaffolds (HepG2) are fabricated successfully, exhibiting high cell proliferation and functionality. The strategy established in this study promotes the printability and operability of light-based 3D bioprinting systems, allowing numerous new applications for tissue engineering.

Date: 2023
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DOI: 10.1038/s41467-023-38838-2

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