A highly sensitive strategy for monitoring real-time proliferation of targeted cell types in vivo

Hiroto Sugawara, Junta Imai (), Junpei Yamamoto, Tomohito Izumi, Yohei Kawana, Akira Endo, Masato Kohata, Junro Seike, Haremaru Kubo, Hiroshi Komamura, Yuichiro Munakata, Yoichiro Asai, Shinichiro Hosaka, Shojiro Sawada, Shinjiro Kodama, Kei Takahashi, Keizo Kaneko and Hideki Katagiri
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Hiroto Sugawara: Tohoku University Graduate School of Medicine
Junta Imai: Tohoku University Graduate School of Medicine
Junpei Yamamoto: Tohoku University Graduate School of Medicine
Tomohito Izumi: Tohoku University Graduate School of Medicine
Yohei Kawana: Tohoku University Graduate School of Medicine
Akira Endo: Tohoku University Graduate School of Medicine
Masato Kohata: Tohoku University Graduate School of Medicine
Junro Seike: Tohoku University Graduate School of Medicine
Haremaru Kubo: Tohoku University Graduate School of Medicine
Hiroshi Komamura: Tohoku University Graduate School of Medicine
Yuichiro Munakata: Tohoku Medical and Pharmaceutical University
Yoichiro Asai: Tohoku University Graduate School of Medicine
Shinichiro Hosaka: Tohoku University Graduate School of Medicine
Shojiro Sawada: Tohoku Medical and Pharmaceutical University
Shinjiro Kodama: Tohoku University Graduate School of Medicine
Kei Takahashi: Tohoku University Graduate School of Medicine
Keizo Kaneko: Tohoku University Graduate School of Medicine
Hideki Katagiri: Tohoku University Graduate School of Medicine

Nature Communications, 2023, vol. 14, issue 1, 1-14

Abstract: Abstract Cell proliferation processes play pivotal roles in timely adaptation to many biological situations. Herein, we establish a highly sensitive and simple strategy by which time-series showing the proliferation of a targeted cell type can be quantitatively monitored in vivo in the same individuals. We generate mice expressing a secreted type of luciferase only in cells producing Cre under the control of the Ki67 promoter. Crossing these with tissue-specific Cre-expressing mice allows us to monitor the proliferation time course of pancreatic β-cells, which are few in number and weakly proliferative, by measuring plasma luciferase activity. Physiological time courses, during obesity development, pregnancy and juvenile growth, as well as diurnal variation, of β-cell proliferation, are clearly detected. Moreover, this strategy can be utilized for highly sensitive ex vivo screening for proliferative factors for targeted cells. Thus, these technologies may contribute to advancements in broad areas of biological and medical research.

Date: 2023
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DOI: 10.1038/s41467-023-38897-5

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