Co-translational binding of importins to nascent proteins

Maximilian Seidel, Natalie Romanov, Agnieszka Obarska-Kosinska, Anja Becker, Nayara Trevisan Doimo de Azevedo, Jan Provaznik, Sankarshana R. Nagaraja, Jonathan J. M. Landry, Vladimir Benes and Martin Beck ()
Additional contact information
Maximilian Seidel: Max Planck Institute of Biophysics
Natalie Romanov: Max Planck Institute of Biophysics
Agnieszka Obarska-Kosinska: Max Planck Institute of Biophysics
Anja Becker: Max Planck Institute of Biophysics
Nayara Trevisan Doimo de Azevedo: European Molecular Biology Laboratory (EMBL)
Jan Provaznik: European Molecular Biology Laboratory (EMBL)
Sankarshana R. Nagaraja: Max Planck Institute of Biophysics
Jonathan J. M. Landry: European Molecular Biology Laboratory (EMBL)
Vladimir Benes: European Molecular Biology Laboratory (EMBL)
Martin Beck: Max Planck Institute of Biophysics

Nature Communications, 2023, vol. 14, issue 1, 1-15

Abstract: Abstract Various cellular quality control mechanisms support proteostasis. While, ribosome-associated chaperones prevent the misfolding of nascent chains during translation, importins were shown to prevent the aggregation of specific cargoes in a post-translational mechanism prior the import into the nucleoplasm. Here, we hypothesize that importins may already bind ribosome-associated cargo in a co-translational manner. We systematically measure the nascent chain association of all importins in Saccharomyces cerevisiae by selective ribosome profiling. We identify a subset of importins that bind to a wide range of nascent, often uncharacterized cargoes. This includes ribosomal proteins, chromatin remodelers and RNA binding proteins that are aggregation prone in the cytosol. We show that importins act consecutively with other ribosome-associated chaperones. Thus, the nuclear import system is directly intertwined with nascent chain folding and chaperoning.

Date: 2023
References: View references in EconPapers View complete reference list from CitEc
Citations:

Downloads: (external link)
https://www.nature.com/articles/s41467-023-39150-9 Abstract (text/html)

Related works:
This item may be available elsewhere in EconPapers: Search for items with the same title.

Export reference: BibTeX RIS (EndNote, ProCite, RefMan) HTML/Text

Persistent link: https://EconPapers.repec.org/RePEc:nat:natcom:v:14:y:2023:i:1:d:10.1038_s41467-023-39150-9

Ordering information: This journal article can be ordered from
https://www.nature.com/ncomms/

DOI: 10.1038/s41467-023-39150-9

Access Statistics for this article

Nature Communications is currently edited by Nathalie Le Bot, Enda Bergin and Fiona Gillespie

More articles in Nature Communications from Nature
Bibliographic data for series maintained by Sonal Shukla () and Springer Nature Abstracting and Indexing ().