Structure and proposed DNA delivery mechanism of a marine roseophage

Yang Huang, Hui Sun, Shuzhen Wei, Lanlan Cai, Liqin Liu, Yanan Jiang, Jiabao Xin, Zhenqin Chen, Yuqiong Que, Zhibo Kong, Tingting Li, Hai Yu, Jun Zhang, Ying Gu, Qingbing Zheng (), Shaowei Li (), Rui Zhang () and Ningshao Xia ()
Additional contact information
Yang Huang: Xiamen University
Hui Sun: Xiamen University
Shuzhen Wei: Xiamen University
Lanlan Cai: The Hong Kong University of Science and Technology
Liqin Liu: Xiamen University
Yanan Jiang: Xiamen University
Jiabao Xin: Xiamen University
Zhenqin Chen: Xiamen University
Yuqiong Que: Xiamen University
Zhibo Kong: Xiamen University
Tingting Li: Xiamen University
Hai Yu: Xiamen University
Jun Zhang: Xiamen University
Ying Gu: Xiamen University
Qingbing Zheng: Xiamen University
Shaowei Li: Xiamen University
Rui Zhang: Xiamen University
Ningshao Xia: Xiamen University

Nature Communications, 2023, vol. 14, issue 1, 1-15

Abstract: Abstract Tailed bacteriophages (order, Caudovirales) account for the majority of all phages. However, the long flexible tail of siphophages hinders comprehensive investigation of the mechanism of viral gene delivery. Here, we report the atomic capsid and in-situ structures of the tail machine of the marine siphophage, vB_DshS-R4C (R4C), which infects Roseobacter. The R4C virion, comprising 12 distinct structural protein components, has a unique five-fold vertex of the icosahedral capsid that allows genome delivery. The specific position and interaction pattern of the tail tube proteins determine the atypical long rigid tail of R4C, and further provide negative charge distribution within the tail tube. A ratchet mechanism assists in DNA transmission, which is initiated by an absorption device that structurally resembles the phage-like particle, RcGTA. Overall, these results provide in-depth knowledge into the intact structure and underlining DNA delivery mechanism for the ecologically important siphophages.

Date: 2023
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DOI: 10.1038/s41467-023-39220-y

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