Maternal NAT10 orchestrates oocyte meiotic cell-cycle progression and maturation in mice

Xue Jiang, Yu Cheng, Yuzhang Zhu, Caoling Xu, Qiaodan Li, Xuemei Xing, Wenqing Li, Jiaqi Zou, Lan Meng, Muhammad Azhar, Yuzhu Cao, Xianhong Tong, Weibing Qin (), Xiaoli Zhu () and Jianqiang Bao ()
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Xue Jiang: University of Science and Technology of China (USTC)
Yu Cheng: University of Science and Technology of China (USTC)
Yuzhang Zhu: University of Science and Technology of China (USTC)
Caoling Xu: University of Science and Technology of China (USTC)
Qiaodan Li: University of Science and Technology of China (USTC)
Xuemei Xing: University of Science and Technology of China (USTC)
Wenqing Li: University of Science and Technology of China (USTC)
Jiaqi Zou: University of Science and Technology of China (USTC)
Lan Meng: University of Science and Technology of China (USTC)
Muhammad Azhar: University of Science and Technology of China (USTC)
Yuzhu Cao: University of Science and Technology of China (USTC)
Xianhong Tong: University of Science and Technology of China (USTC)
Weibing Qin: Guangdong Provincial Reproductive Science Institute (Guangdong Provincial Fertility Hospital)
Xiaoli Zhu: University of Science and Technology of China (USTC)
Jianqiang Bao: University of Science and Technology of China (USTC)

Nature Communications, 2023, vol. 14, issue 1, 1-23

Abstract: Abstract In mammals, the production of mature oocytes necessitates rigorous regulation of the discontinuous meiotic cell-cycle progression at both the transcriptional and post-transcriptional levels. However, the factors underlying this sophisticated but explicit process remain largely unclear. Here we characterize the function of N-acetyltransferase 10 (Nat10), a writer for N4-acetylcytidine (ac4C) on RNA molecules, in mouse oocyte development. We provide genetic evidence that Nat10 is essential for oocyte meiotic prophase I progression, oocyte growth and maturation by sculpting the maternal transcriptome through timely degradation of poly(A) tail mRNAs. This is achieved through the ac4C deposition on the key CCR4-NOT complex transcripts. Importantly, we devise a method for examining the poly(A) tail length (PAT), termed Hairpin Adaptor-poly(A) tail length (HA-PAT), which outperforms conventional methods in terms of cost, sensitivity, and efficiency. In summary, these findings provide genetic evidence that unveils the indispensable role of maternal Nat10 in oocyte development.

Date: 2023
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DOI: 10.1038/s41467-023-39256-0

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