PPP2R1A regulates migration persistence through the NHSL1-containing WAVE Shell Complex

Yanan Wang, Giovanni Chiappetta, Raphaël Guérois, Yijun Liu, Stéphane Romero, Daniel J. Boesch, Matthias Krause, Claire A. Dessalles, Avin Babataheri, Abdul I. Barakat, Baoyu Chen, Joelle Vinh, Anna Polesskaya () and Alexis M. Gautreau ()
Additional contact information
Yanan Wang: Institut Polytechnique de Paris
Giovanni Chiappetta: Université PSL
Raphaël Guérois: Université Paris-Saclay, CEA, CNRS, Institute for Integrative Biology of the Cell (I2BC)
Yijun Liu: Iowa State University
Stéphane Romero: Institut Polytechnique de Paris
Daniel J. Boesch: Iowa State University
Matthias Krause: King’s College London
Claire A. Dessalles: Institut Polytechnique de Paris
Avin Babataheri: Institut Polytechnique de Paris
Abdul I. Barakat: Institut Polytechnique de Paris
Baoyu Chen: Iowa State University
Joelle Vinh: Université PSL
Anna Polesskaya: Institut Polytechnique de Paris
Alexis M. Gautreau: Institut Polytechnique de Paris

Nature Communications, 2023, vol. 14, issue 1, 1-18

Abstract: Abstract The RAC1-WAVE-Arp2/3 signaling pathway generates branched actin networks that power lamellipodium protrusion of migrating cells. Feedback is thought to control protrusion lifetime and migration persistence, but its molecular circuitry remains elusive. Here, we identify PPP2R1A by proteomics as a protein differentially associated with the WAVE complex subunit ABI1 when RAC1 is activated and downstream generation of branched actin is blocked. PPP2R1A is found to associate at the lamellipodial edge with an alternative form of WAVE complex, the WAVE Shell Complex, that contains NHSL1 instead of the Arp2/3 activating subunit WAVE, as in the canonical WAVE Regulatory Complex. PPP2R1A is required for persistence in random and directed migration assays and for RAC1-dependent actin polymerization in cell extracts. PPP2R1A requirement is abolished by NHSL1 depletion. PPP2R1A mutations found in tumors impair WAVE Shell Complex binding and migration regulation, suggesting that the coupling of PPP2R1A to the WAVE Shell Complex is essential to its function.

Date: 2023
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DOI: 10.1038/s41467-023-39276-w

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