Selective binding of retrotransposons by ZFP352 facilitates the timely dissolution of totipotency network

Zhengyi Li, Haiyan Xu, Jiaqun Li, Xiao Xu, Junjiao Wang, Danya Wu, Jiateng Zhang, Juan Liu, Ziwei Xue, Guankai Zhan, Bobby Cheng Peow Tan, Di Chen, Yun-Shen Chan, Huck Hui Ng, Wanlu Liu, Chih-Hung Hsu, Dan Zhang (), Yang Shen () and Hongqing Liang ()
Additional contact information
Zhengyi Li: Zhejiang University School of Medicine
Haiyan Xu: Zhejiang University School of Medicine
Jiaqun Li: Zhejiang University
Xiao Xu: Zhejiang University School of Medicine
Junjiao Wang: Zhejiang University School of Medicine
Danya Wu: Zhejiang University School of Medicine
Jiateng Zhang: Zhejiang University School of Medicine
Juan Liu: Zhejiang University
Ziwei Xue: Zhejiang University
Guankai Zhan: Zhejiang University School of Medicine
Bobby Cheng Peow Tan: Agency for Science, Technology and Research (A*STAR)
Di Chen: Zhejiang University
Yun-Shen Chan: Guangzhou International Bio Island
Huck Hui Ng: Agency for Science, Technology and Research (A*STAR)
Wanlu Liu: Zhejiang University
Chih-Hung Hsu: Zhejiang University School of Medicine
Dan Zhang: Zhejiang University
Yang Shen: Agency for Science, Technology and Research (A*STAR)
Hongqing Liang: Zhejiang University School of Medicine

Nature Communications, 2023, vol. 14, issue 1, 1-16

Abstract: Abstract Acquisition of new stem cell fates relies on the dissolution of the prior regulatory network sustaining the existing cell fates. Currently, extensive insights have been revealed for the totipotency regulatory network around the zygotic genome activation (ZGA) period. However, how the dissolution of the totipotency network is triggered to ensure the timely embryonic development following ZGA is largely unknown. In this study, we identify the unexpected role of a highly expressed 2-cell (2C) embryo specific transcription factor, ZFP352, in facilitating the dissolution of the totipotency network. We find that ZFP352 has selective binding towards two different retrotransposon sub-families. ZFP352 coordinates with DUX to bind the 2C specific MT2_Mm sub-family. On the other hand, without DUX, ZFP352 switches affinity to bind extensively onto SINE_B1/Alu sub-family. This leads to the activation of later developmental programs like ubiquitination pathways, to facilitate the dissolution of the 2C state. Correspondingly, depleting ZFP352 in mouse embryos delays the 2C to morula transition process. Thus, through a shift of binding from MT2_Mm to SINE_B1/Alu, ZFP352 can trigger spontaneous dissolution of the totipotency network. Our study highlights the importance of different retrotransposons sub-families in facilitating the timely and programmed cell fates transition during early embryogenesis.

Date: 2023
References: View references in EconPapers View complete reference list from CitEc
Citations:

Downloads: (external link)
https://www.nature.com/articles/s41467-023-39344-1 Abstract (text/html)

Related works:
This item may be available elsewhere in EconPapers: Search for items with the same title.

Export reference: BibTeX RIS (EndNote, ProCite, RefMan) HTML/Text

Persistent link: https://EconPapers.repec.org/RePEc:nat:natcom:v:14:y:2023:i:1:d:10.1038_s41467-023-39344-1

Ordering information: This journal article can be ordered from
https://www.nature.com/ncomms/

DOI: 10.1038/s41467-023-39344-1

Access Statistics for this article

Nature Communications is currently edited by Nathalie Le Bot, Enda Bergin and Fiona Gillespie

More articles in Nature Communications from Nature
Bibliographic data for series maintained by Sonal Shukla () and Springer Nature Abstracting and Indexing ().