Phase intensity nanoscope (PINE) opens long-time investigation windows of living matter

Guangjie Cui, Yunbo Liu, Di Zu, Xintao Zhao, Zhijia Zhang, Do Young Kim, Pramith Senaratne, Aaron Fox, David Sept, Younggeun Park and Somin Eunice Lee ()
Additional contact information
Guangjie Cui: University of Michigan
Yunbo Liu: University of Michigan
Di Zu: University of Michigan
Xintao Zhao: University of Michigan
Zhijia Zhang: University of Michigan
Do Young Kim: University of Michigan
Pramith Senaratne: University of Michigan
Aaron Fox: University of Michigan
David Sept: University of Michigan
Younggeun Park: University of Michigan
Somin Eunice Lee: University of Michigan

Nature Communications, 2023, vol. 14, issue 1, 1-10

Abstract: Abstract Fundamental to all living organisms and living soft matter are emergent processes in which the reorganization of individual constituents at the nanoscale drives group-level movements and shape changes at the macroscale over time. However, light-induced degradation of fluorophores, photobleaching, is a significant problem in extended bioimaging in life science. Here, we report opening a long-time investigation window by nonbleaching phase intensity nanoscope: PINE. We accomplish phase-intensity separation such that nanoprobe distributions are distinguished by an integrated phase-intensity multilayer thin film (polyvinyl alcohol/liquid crystal). We overcame a physical limit to resolve sub-10 nm cellular architectures, and achieve the first dynamic imaging of nanoscopic reorganization over 250 h using PINE. We discover nanoscopic rearrangements synchronized with the emergence of group-level movements and shape changes at the macroscale according to a set of interaction rules with importance in cellular and soft matter reorganization, self-organization, and pattern formation.

Date: 2023
References: View references in EconPapers View complete reference list from CitEc
Citations:

Downloads: (external link)
https://www.nature.com/articles/s41467-023-39624-w Abstract (text/html)

Related works:
This item may be available elsewhere in EconPapers: Search for items with the same title.

Export reference: BibTeX RIS (EndNote, ProCite, RefMan) HTML/Text

Persistent link: https://EconPapers.repec.org/RePEc:nat:natcom:v:14:y:2023:i:1:d:10.1038_s41467-023-39624-w

Ordering information: This journal article can be ordered from
https://www.nature.com/ncomms/

DOI: 10.1038/s41467-023-39624-w

Access Statistics for this article

Nature Communications is currently edited by Nathalie Le Bot, Enda Bergin and Fiona Gillespie

More articles in Nature Communications from Nature
Bibliographic data for series maintained by Sonal Shukla () and Springer Nature Abstracting and Indexing ().