High-speed scanless entire bandwidth mid-infrared chemical imaging

Yue Zhao (), Shota Kusama, Yuji Furutani, Wei-Hong Huang, Chih-Wei Luo and Takao Fuji ()
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Yue Zhao: Toyota Technological Institute
Shota Kusama: Toyota Technological Institute
Yuji Furutani: Nagoya Institute of Technology, Showa-Ku
Wei-Hong Huang: National Yang Ming Chiao Tung University
Chih-Wei Luo: National Yang Ming Chiao Tung University
Takao Fuji: Toyota Technological Institute

Nature Communications, 2023, vol. 14, issue 1, 1-11

Abstract: Abstract Mid-infrared spectroscopy probes molecular vibrations to identify chemical species and functional groups. Therefore, mid-infrared hyperspectral imaging is one of the most powerful and promising candidates for chemical imaging using optical methods. Yet high-speed and entire bandwidth mid-infrared hyperspectral imaging has not been realized. Here we report a mid-infrared hyperspectral chemical imaging technique that uses chirped pulse upconversion of sub-cycle pulses at the image plane. This technique offers a lateral resolution of 15 µm, and the field of view is adjustable between 800 µm × 600 µm to 12 mm × 9 mm. The hyperspectral imaging produces a 640 × 480 pixel image in 8 s, which covers a spectral range of 640–3015 cm–1, comprising 1069 wavelength points and offering a wavenumber resolution of 2.6–3.7 cm–1. For discrete frequency mid-infrared imaging, the measurement speed reaches a frame rate of 5 kHz, the repetition rate of the laser. As a demonstration, we effectively identified and mapped different components in a microfluidic device, plant cell, and mouse embryo section. The great capacity and latent force of this technique in chemical imaging promise to be applied to many fields such as chemical analysis, biology, and medicine.

Date: 2023
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DOI: 10.1038/s41467-023-39628-6

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