ASH1L-MRG15 methyltransferase deposits H3K4me3 and FACT for damage verification in nucleotide excision repair

Corina Maritz, Reihaneh Khaleghi, Michelle N. Yancoskie, Sarah Diethelm, Sonja Brülisauer, Natalia Santos Ferreira, Yang Jiang, Shana J. Sturla and Hanspeter Naegeli ()
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Corina Maritz: University of Zurich-Vetsuisse
Reihaneh Khaleghi: University of Zurich-Vetsuisse
Michelle N. Yancoskie: University of Zurich-Vetsuisse
Sarah Diethelm: University of Zurich-Vetsuisse
Sonja Brülisauer: University of Zurich-Vetsuisse
Natalia Santos Ferreira: University of Zurich-Vetsuisse
Yang Jiang: ETH Zurich
Shana J. Sturla: ETH Zurich
Hanspeter Naegeli: University of Zurich-Vetsuisse

Nature Communications, 2023, vol. 14, issue 1, 1-16

Abstract: Abstract To recognize DNA adducts, nucleotide excision repair (NER) deploys the XPC sensor, which detects damage-induced helical distortions, followed by engagement of TFIIH for lesion verification. Accessory players ensure that this factor handover takes place in chromatin where DNA is tightly wrapped around histones. Here, we describe how the histone methyltransferase ASH1L, once activated by MRG15, helps XPC and TFIIH to navigate through chromatin and induce global-genome NER hotspots. Upon UV irradiation, ASH1L adds H3K4me3 all over the genome (except in active gene promoters), thus priming chromatin for XPC relocations from native to damaged DNA. The ASH1L-MRG15 complex further recruits the histone chaperone FACT to DNA lesions. In the absence of ASH1L, MRG15 or FACT, XPC is misplaced and persists on damaged DNA without being able to deliver the lesions to TFIIH. We conclude that ASH1L-MRG15 makes damage verifiable by the NER machinery through the sequential deposition of H3K4me3 and FACT.

Date: 2023
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DOI: 10.1038/s41467-023-39635-7

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