Epigenetic mechanisms to propagate histone acetylation by p300/CBP

Masaki Kikuchi, Satoshi Morita, Masatoshi Wakamori, Shin Sato, Tomomi Uchikubo-Kamo, Takehiro Suzuki, Naoshi Dohmae, Mikako Shirouzu and Takashi Umehara ()
Additional contact information
Masaki Kikuchi: RIKEN Center for Biosystems Dynamics Research
Satoshi Morita: RIKEN Center for Biosystems Dynamics Research
Masatoshi Wakamori: RIKEN Center for Biosystems Dynamics Research
Shin Sato: RIKEN Center for Biosystems Dynamics Research
Tomomi Uchikubo-Kamo: RIKEN Center for Biosystems Dynamics Research
Takehiro Suzuki: Technology Platform Division, RIKEN Center for Sustainable Resource Science
Naoshi Dohmae: Technology Platform Division, RIKEN Center for Sustainable Resource Science
Mikako Shirouzu: RIKEN Center for Biosystems Dynamics Research
Takashi Umehara: RIKEN Center for Biosystems Dynamics Research

Nature Communications, 2023, vol. 14, issue 1, 1-16

Abstract: Abstract Histone acetylation is important for the activation of gene transcription but little is known about its direct read/write mechanisms. Here, we report cryogenic electron microscopy structures in which a p300/CREB-binding protein (CBP) multidomain monomer recognizes histone H4 N-terminal tail (NT) acetylation (ac) in a nucleosome and acetylates non-H4 histone NTs within the same nucleosome. p300/CBP not only recognized H4NTac via the bromodomain pocket responsible for reading, but also interacted with the DNA minor grooves via the outside of that pocket. This directed the catalytic center of p300/CBP to one of the non-H4 histone NTs. The primary target that p300 writes by reading H4NTac was H2BNT, and H2BNTac promoted H2A-H2B dissociation from the nucleosome. We propose a model in which p300/CBP replicates histone N-terminal tail acetylation within the H3-H4 tetramer to inherit epigenetic storage, and transcribes it from the H3-H4 tetramer to the H2B-H2A dimers to activate context-dependent gene transcription through local nucleosome destabilization.

Date: 2023
References: View references in EconPapers View complete reference list from CitEc
Citations: View citations in EconPapers (1)

Downloads: (external link)
https://www.nature.com/articles/s41467-023-39735-4 Abstract (text/html)

Related works:
This item may be available elsewhere in EconPapers: Search for items with the same title.

Export reference: BibTeX RIS (EndNote, ProCite, RefMan) HTML/Text

Persistent link: https://EconPapers.repec.org/RePEc:nat:natcom:v:14:y:2023:i:1:d:10.1038_s41467-023-39735-4

Ordering information: This journal article can be ordered from
https://www.nature.com/ncomms/

DOI: 10.1038/s41467-023-39735-4

Access Statistics for this article

Nature Communications is currently edited by Nathalie Le Bot, Enda Bergin and Fiona Gillespie

More articles in Nature Communications from Nature
Bibliographic data for series maintained by Sonal Shukla () and Springer Nature Abstracting and Indexing ().