Maternal TDP-43 interacts with RNA Pol II and regulates zygotic genome activation

Xiaoqing Nie, Qianhua Xu, Chengpeng Xu, Fengling Chen, Qizhi Wang, Dandan Qin, Rui Wang, Zheng Gao, Xukun Lu, Xinai Yang, Yu Wu, Chen Gu, Wei Xie () and Lei Li ()
Additional contact information
Xiaoqing Nie: Chinese Academy of Sciences
Qianhua Xu: Tsinghua University
Chengpeng Xu: Chinese Academy of Sciences
Fengling Chen: Tsinghua University
Qizhi Wang: Chinese Academy of Sciences
Dandan Qin: Chinese Academy of Sciences
Rui Wang: Chinese Academy of Sciences
Zheng Gao: Reproductive Medicine Center of the Third Affiliated Hospital of Guangzhou Medical University
Xukun Lu: Tsinghua University
Xinai Yang: Chinese Academy of Sciences
Yu Wu: Chinese Academy of Sciences
Chen Gu: Chinese Academy of Sciences
Wei Xie: Tsinghua University
Lei Li: Chinese Academy of Sciences

Nature Communications, 2023, vol. 14, issue 1, 1-12

Abstract: Abstract Zygotic genome activation (ZGA) is essential for early embryonic development. However, the regulation of ZGA remains elusive in mammals. Here we report that a maternal factor TDP-43, a nuclear transactive response DNA-binding protein, regulates ZGA through RNA Pol II and is essential for mouse early embryogenesis. Maternal TDP-43 translocates from the cytoplasm into the nucleus at the early two-cell stage when minor to major ZGA transition occurs. Genetic deletion of maternal TDP-43 results in mouse early embryos arrested at the two-cell stage. TDP-43 co-occupies with RNA Pol II as large foci in the nucleus and also at the promoters of ZGA genes at the late two-cell stage. Biochemical evidence indicates that TDP-43 binds Polr2a and Cyclin T1. Depletion of maternal TDP-43 caused the loss of Pol II foci and reduced Pol II binding on chromatin at major ZGA genes, accompanied by defective ZGA. Collectively, our results suggest that maternal TDP-43 is critical for mouse early embryonic development, in part through facilitating the correct RNA Pol II configuration and zygotic genome activation.

Date: 2023
References: View references in EconPapers View complete reference list from CitEc
Citations:

Downloads: (external link)
https://www.nature.com/articles/s41467-023-39924-1 Abstract (text/html)

Related works:
This item may be available elsewhere in EconPapers: Search for items with the same title.

Export reference: BibTeX RIS (EndNote, ProCite, RefMan) HTML/Text

Persistent link: https://EconPapers.repec.org/RePEc:nat:natcom:v:14:y:2023:i:1:d:10.1038_s41467-023-39924-1

Ordering information: This journal article can be ordered from
https://www.nature.com/ncomms/

DOI: 10.1038/s41467-023-39924-1

Access Statistics for this article

Nature Communications is currently edited by Nathalie Le Bot, Enda Bergin and Fiona Gillespie

More articles in Nature Communications from Nature
Bibliographic data for series maintained by Sonal Shukla () and Springer Nature Abstracting and Indexing ().