Deep-profiling of phospholipidome via rapid orthogonal separations and isomer-resolved mass spectrometry

Tian Xia, Feng Zhou, Donghui Zhang, Xue Jin, Hengxue Shi, Hang Yin, Yanqing Gong and Yu Xia ()
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Tian Xia: Tsinghua University
Feng Zhou: Bytedance Technology Co.
Donghui Zhang: Tsinghua University, Department of Precision Instrument
Xue Jin: Tsinghua University
Hengxue Shi: Tsinghua University
Hang Yin: Tsinghua University
Yanqing Gong: Peking University First Hospital
Yu Xia: Tsinghua University

Nature Communications, 2023, vol. 14, issue 1, 1-12

Abstract: Abstract A lipidome comprises thousands of lipid species, many of which are isomers and isobars. Liquid chromatography-tandem mass spectrometry (LC-MS/MS), although widely used for lipidomic profiling, faces challenges in differentiating lipid isomers. Herein, we address this issue by leveraging the orthogonal separation capabilities of hydrophilic interaction liquid chromatography (HILIC) and trapped ion mobility spectrometry (TIMS). We further integrate isomer-resolved MS/MS methods onto HILIC-TIMS, which enable pinpointing double bond locations in phospholipids and sn-positions in phosphatidylcholine. This system profiles phospholipids at multiple structural levels with short analysis time (

Date: 2023
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DOI: 10.1038/s41467-023-40046-x

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