Dual-color DNA-PAINT single-particle tracking enables extended studies of membrane protein interactions

Christian Niederauer, Chikim Nguyen, Miles Wang-Henderson, Johannes Stein, Sebastian Strauss, Alexander Cumberworth, Florian Stehr, Ralf Jungmann, Petra Schwille and Kristina A. Ganzinger ()
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Christian Niederauer: AMOLF
Chikim Nguyen: AMOLF
Miles Wang-Henderson: AMOLF
Johannes Stein: Max Planck Institute of Biochemistry
Sebastian Strauss: Max Planck Institute of Biochemistry
Alexander Cumberworth: AMOLF
Florian Stehr: Max Planck Institute of Biochemistry
Ralf Jungmann: Max Planck Institute of Biochemistry
Petra Schwille: Max Planck Institute of Biochemistry
Kristina A. Ganzinger: AMOLF

Nature Communications, 2023, vol. 14, issue 1, 1-8

Abstract: Abstract DNA-PAINT based single-particle tracking (DNA-PAINT-SPT) has recently significantly enhanced observation times in in vitro SPT experiments by overcoming the constraints of fluorophore photobleaching. However, with the reported implementation, only a single target can be imaged and the technique cannot be applied straight to live cell imaging. Here we report on leveraging this technique from a proof-of-principle implementation to a useful tool for the SPT community by introducing simultaneous live cell dual-color DNA-PAINT-SPT for quantifying protein dimerization and tracking proteins in living cell membranes, demonstrating its improved performance over single-dye SPT.

Date: 2023
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DOI: 10.1038/s41467-023-40065-8

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