Long-term ex situ normothermic perfusion of human split livers for more than 1 week

Ngee-Soon Lau, Mark Ly, Claude Dennis, Andrew Jacques, Marti Cabanes-Creus, Shamus Toomath, Joanna Huang, Nicole Mestrovic, Paul Yousif, Sumon Chanda, Chuanmin Wang, Leszek Lisowski, Ken Liu, James G. Kench, Geoffrey McCaughan, Michael Crawford and Carlo Pulitano ()
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Ngee-Soon Lau: Royal Prince Alfred Hospital
Mark Ly: Royal Prince Alfred Hospital
Claude Dennis: Royal Prince Alfred Hospital
Andrew Jacques: Royal Prince Alfred Hospital
Marti Cabanes-Creus: The University of Sydney, Westmead
Shamus Toomath: Royal Prince Alfred Hospital
Joanna Huang: Royal Prince Alfred Hospital
Nicole Mestrovic: Royal Prince Alfred Hospital
Paul Yousif: Royal Prince Alfred Hospital
Sumon Chanda: Royal Prince Alfred Hospital
Chuanmin Wang: Royal Prince Alfred Hospital
Leszek Lisowski: The University of Sydney, Westmead
Ken Liu: Royal Prince Alfred Hospital
James G. Kench: The University of Sydney
Geoffrey McCaughan: Royal Prince Alfred Hospital
Michael Crawford: Royal Prince Alfred Hospital
Carlo Pulitano: Royal Prince Alfred Hospital

Nature Communications, 2023, vol. 14, issue 1, 1-11

Abstract: Abstract Current machine perfusion technology permits livers to be preserved ex situ for short periods to assess viability prior to transplant. Long-term normothermic perfusion of livers is an emerging field with tremendous potential for the assessment, recovery, and modification of organs. In this study, we aimed to develop a long-term model of ex situ perfusion including a surgical split and simultaneous perfusion of both partial organs. Human livers declined for transplantation were perfused using a red blood cell-based perfusate under normothermic conditions (36 °C) and then split and simultaneously perfused on separate machines. Ten human livers were split, resulting in 20 partial livers. The median ex situ viability was 125 h, and the median ex situ survival was 165 h. Long-term survival was demonstrated by lactate clearance, bile production, Factor-V production, and storage of adenosine triphosphate. Here, we report the long-term ex situ perfusion of human livers and demonstrate the ability to split and perfuse these organs using a standardised protocol.

Date: 2023
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DOI: 10.1038/s41467-023-40154-8

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