Discovery and rational engineering of PET hydrolase with both mesophilic and thermophilic PET hydrolase properties

Hwaseok Hong, Dongwoo Ki, Hogyun Seo, Jiyoung Park, Jaewon Jang and Kyung-Jin Kim ()
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Hwaseok Hong: Kyungpook National University
Dongwoo Ki: Kyungpook National University
Hogyun Seo: Kyungpook National University
Jiyoung Park: Kyungpook National University
Jaewon Jang: CJ CheilJedang Co.
Kyung-Jin Kim: Kyungpook National University

Nature Communications, 2023, vol. 14, issue 1, 1-13

Abstract: Abstract Excessive polyethylene terephthalate (PET) waste causes a variety of problems. Extensive research focused on the development of superior PET hydrolases for PET biorecycling has been conducted. However, template enzymes employed in enzyme engineering mainly focused on IsPETase and leaf-branch compost cutinase, which exhibit mesophilic and thermophilic hydrolytic properties, respectively. Herein, we report a PET hydrolase from Cryptosporangium aurantiacum (CaPETase) that exhibits high thermostability and remarkable PET degradation activity at ambient temperatures. We uncover the crystal structure of CaPETase, which displays a distinct backbone conformation at the active site and residues forming the substrate binding cleft, compared with other PET hydrolases. We further develop a CaPETaseM9 variant that exhibits robust thermostability with a Tm of 83.2 °C and 41.7-fold enhanced PET hydrolytic activity at 60 °C compared with CaPETaseWT. CaPETaseM9 almost completely decompose both transparent and colored post-consumer PET powder at 55 °C within half a day in a pH-stat bioreactor.

Date: 2023
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DOI: 10.1038/s41467-023-40233-w

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