Click-electrochemistry for the rapid labeling of virus, bacteria and cell surfaces

Sébastien Depienne, Mohammed Bouzelha, Emmanuelle Courtois, Karine Pavageau, Pierre-Alban Lalys, Maia Marchand, Dimitri Alvarez-Dorta, Steven Nedellec, Laura Marín-Fernández, Cyrille Grandjean, Mohammed Boujtita, David Deniaud, Mathieu Mével () and Sébastien G. Gouin ()
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Sébastien Depienne: Nantes Université, CNRS, CEISAM UMR 6230
Mohammed Bouzelha: Nantes Université, TaRGeT, Translational Research for Gene Therapies, CHU Nantes, INSERM, UMR 1089
Emmanuelle Courtois: Nantes Université, CNRS, US2B, UMR 6286
Karine Pavageau: Nantes Université, TaRGeT, Translational Research for Gene Therapies, CHU Nantes, INSERM, UMR 1089
Pierre-Alban Lalys: Nantes Université, CNRS, CEISAM UMR 6230
Maia Marchand: Nantes Université, CNRS, CEISAM UMR 6230
Dimitri Alvarez-Dorta: Nantes Université, CNRS, CEISAM UMR 6230
Steven Nedellec: Nantes Université, CHU Nantes, CNRS, Inserm, BioCore, US16, SFR Bonamy
Laura Marín-Fernández: Nantes Université, CNRS, US2B, UMR 6286
Cyrille Grandjean: Nantes Université, CNRS, US2B, UMR 6286
Mohammed Boujtita: Nantes Université, CNRS, CEISAM UMR 6230
David Deniaud: Nantes Université, CNRS, CEISAM UMR 6230
Mathieu Mével: Nantes Université, TaRGeT, Translational Research for Gene Therapies, CHU Nantes, INSERM, UMR 1089
Sébastien G. Gouin: Nantes Université, CNRS, CEISAM UMR 6230

Nature Communications, 2023, vol. 14, issue 1, 1-10

Abstract: Abstract Methods for direct covalent ligation of microorganism surfaces remain poorly reported, and mostly based on metabolic engineering for bacteria and cells functionalization. While effective, a faster method avoiding the bio-incorporation step would be highly complementary. Here, we used N-methylluminol (NML), a fully tyrosine-selective protein anchoring group after one-electron oxidation, to label the surface of viruses, living bacteria and cells. The functionalization was performed electrochemically and in situ by applying an electric potential to aqueous buffered solutions of tagged NML containing the viruses, bacteria or cells. The broad applicability of the click-electrochemistry method was explored on recombinant adeno-associated viruses (rAAV2), Escherichia coli (Gram-) and Staphyloccocus epidermidis (Gram + ) bacterial strains, and HEK293 and HeLa eukaryotic cell lines. Surface electro-conjugation was achieved in minutes to yield functionalized rAAV2 that conserved both structural integrity and infectivity properties, and living bacteria and cell lines that were still alive and able to divide.

Date: 2023
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DOI: 10.1038/s41467-023-40534-0

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