An integrated proteome and transcriptome of B cell maturation defines poised activation states of transitional and mature B cells

Fiamma Salerno (), Andrew J. M. Howden, Louise S. Matheson, Özge Gizlenci, Michael Screen, Holger Lingel, Monika C. Brunner-Weinzierl and Martin Turner ()
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Fiamma Salerno: The Babraham Institute
Andrew J. M. Howden: University of Dundee
Louise S. Matheson: The Babraham Institute
Özge Gizlenci: The Babraham Institute
Michael Screen: The Babraham Institute
Holger Lingel: Otto-von-Guericke-University
Monika C. Brunner-Weinzierl: Otto-von-Guericke-University
Martin Turner: The Babraham Institute

Nature Communications, 2023, vol. 14, issue 1, 1-18

Abstract: Abstract During B cell maturation, transitional and mature B cells acquire cell-intrinsic features that determine their ability to exit quiescence and mount effective immune responses. Here we use label-free proteomics to quantify the proteome of B cell subsets from the mouse spleen and map the differential expression of environmental sensing, transcription, and translation initiation factors that define cellular identity and function. Cross-examination of the full-length transcriptome and proteome identifies mRNAs related to B cell activation and antibody secretion that are not accompanied by detection of the encoded proteins. In addition, proteomic data further suggests that the translational repressor PDCD4 restrains B cell responses, in particular those from marginal zone B cells, to a T-cell independent antigen. In summary, our molecular characterization of B cell maturation presents a valuable resource to further explore the mechanisms underpinning the specialized functions of B cell subsets, and suggest the presence of ‘poised’ mRNAs that enable expedited B cell responses.

Date: 2023
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Persistent link: https://EconPapers.repec.org/RePEc:nat:natcom:v:14:y:2023:i:1:d:10.1038_s41467-023-40621-2

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DOI: 10.1038/s41467-023-40621-2

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