Control of intracellular pH and bicarbonate by CO2 diffusion into human sperm

Elena Grahn, Svenja V. Kaufmann, Malika Askarova, Momchil Ninov, Luisa M. Welp, Thomas K. Berger (), Henning Urlaub () and U.Benjamin Kaupp ()
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Elena Grahn: Molecular Sensory Systems
Svenja V. Kaufmann: Bioanalytical Mass Spectrometry
Malika Askarova: Molecular Sensory Systems
Momchil Ninov: Bioanalytical Mass Spectrometry
Luisa M. Welp: Bioanalytical Mass Spectrometry
Thomas K. Berger: Molecular Sensory Systems
Henning Urlaub: Bioanalytical Mass Spectrometry
U.Benjamin Kaupp: Molecular Sensory Systems

Nature Communications, 2023, vol. 14, issue 1, 1-17

Abstract: Abstract The reaction of CO2 with H2O to form bicarbonate (HCO3−) and H+ controls sperm motility and fertilization via HCO3−-stimulated cAMP synthesis. A complex network of signaling proteins participates in this reaction. Here, we identify key players that regulate intracellular pH (pHi) and HCO3− in human sperm by quantitative mass spectrometry (MS) and kinetic patch-clamp fluorometry. The resting pHi is set by amiloride-sensitive Na+/H+ exchange. The sperm-specific putative Na+/H+ exchanger SLC9C1, unlike its sea urchin homologue, is not gated by voltage or cAMP. Transporters and channels implied in HCO3− transport are not detected, and may be present at copy numbers

Date: 2023
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DOI: 10.1038/s41467-023-40855-0

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