DNA nanopores as artificial membrane channels for bioprotonics

Le Luo, Swathi Manda, Yunjeong Park, Busra Demir, Jesse Sanchez, M. P. Anantram, Ersin Emre Oren, Ashwin Gopinath () and Marco Rolandi ()
Additional contact information
Le Luo: University of California, Santa Cruz
Swathi Manda: Massachusetts Institute of Technology
Yunjeong Park: University of California, Santa Cruz
Busra Demir: TOBB University of Economics and Technology
Jesse Sanchez: University of California, Santa Cruz
M. P. Anantram: University of Washington
Ersin Emre Oren: TOBB University of Economics and Technology
Ashwin Gopinath: Massachusetts Institute of Technology
Marco Rolandi: University of California, Santa Cruz

Nature Communications, 2023, vol. 14, issue 1, 1-10

Abstract: Abstract Biological membrane channels mediate information exchange between cells and facilitate molecular recognition. While tuning the shape and function of membrane channels for precision molecular sensing via de-novo routes is complex, an even more significant challenge is interfacing membrane channels with electronic devices for signal readout, which results in low efficiency of information transfer - one of the major barriers to the continued development of high-performance bioelectronic devices. To this end, we integrate membrane spanning DNA nanopores with bioprotonic contacts to create programmable, modular, and efficient artificial ion-channel interfaces. Here we show that cholesterol modified DNA nanopores spontaneously and with remarkable affinity span the lipid bilayer formed over the planar bio-protonic electrode surface and mediate proton transport across the bilayer. Using the ability to easily modify DNA nanostructures, we illustrate that this bioprotonic device can be programmed for electronic recognition of biomolecular signals such as presence of Streptavidin and the cardiac biomarker B-type natriuretic peptide, without modifying the biomolecules. We anticipate this robust interface will allow facile electronic measurement and quantification of biomolecules in a multiplexed manner.

Date: 2023
References: View references in EconPapers View complete reference list from CitEc
Citations:

Downloads: (external link)
https://www.nature.com/articles/s41467-023-40870-1 Abstract (text/html)

Related works:
This item may be available elsewhere in EconPapers: Search for items with the same title.

Export reference: BibTeX RIS (EndNote, ProCite, RefMan) HTML/Text

Persistent link: https://EconPapers.repec.org/RePEc:nat:natcom:v:14:y:2023:i:1:d:10.1038_s41467-023-40870-1

Ordering information: This journal article can be ordered from
https://www.nature.com/ncomms/

DOI: 10.1038/s41467-023-40870-1

Access Statistics for this article

Nature Communications is currently edited by Nathalie Le Bot, Enda Bergin and Fiona Gillespie

More articles in Nature Communications from Nature
Bibliographic data for series maintained by Sonal Shukla () and Springer Nature Abstracting and Indexing ().