A pathogen-induced putative NAC transcription factor mediates leaf rust resistance in barley

Chunhong Chen, Matthias Jost, Megan A. Outram, Dorian Friendship, Jian Chen, Aihua Wang, Sambasivam Periyannan, Jan Bartoš, Kateřina Holušová, Jaroslav Doležel, Peng Zhang, Dhara Bhatt, Davinder Singh, Evans Lagudah (), Robert F. Park () and Peter M. Dracatos ()
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Chunhong Chen: Commonwealth Scientific and Industrial Research Organisation
Matthias Jost: Commonwealth Scientific and Industrial Research Organisation
Megan A. Outram: Commonwealth Scientific and Industrial Research Organisation
Dorian Friendship: The University of Sydney, Faculty of Science, Plant Breeding Institute
Jian Chen: Commonwealth Scientific and Industrial Research Organisation
Aihua Wang: Commonwealth Scientific and Industrial Research Organisation
Sambasivam Periyannan: Commonwealth Scientific and Industrial Research Organisation
Jan Bartoš: Centre of Plant Structural and Functional Genomics
Kateřina Holušová: Centre of Plant Structural and Functional Genomics
Jaroslav Doležel: Centre of Plant Structural and Functional Genomics
Peng Zhang: The University of Sydney, Faculty of Science, Plant Breeding Institute
Dhara Bhatt: Commonwealth Scientific and Industrial Research Organisation
Davinder Singh: The University of Sydney, Faculty of Science, Plant Breeding Institute
Evans Lagudah: Commonwealth Scientific and Industrial Research Organisation
Robert F. Park: The University of Sydney, Faculty of Science, Plant Breeding Institute
Peter M. Dracatos: The University of Sydney, Faculty of Science, Plant Breeding Institute

Nature Communications, 2023, vol. 14, issue 1, 1-11

Abstract: Abstract Leaf rust, caused by Puccinia hordei, is one of the most widespread and damaging foliar diseases affecting barley. The barley leaf rust resistance locus Rph7 has been shown to have unusually high sequence and haplotype divergence. In this study, we isolate the Rph7 gene using a fine mapping and RNA-Seq approach that is confirmed by mutational analysis and transgenic complementation. Rph7 is a pathogen-induced, non-canonical resistance gene encoding a protein that is distinct from other known plant disease resistance proteins in the Triticeae. Structural analysis using an AlphaFold2 protein model suggests that Rph7 encodes a putative NAC transcription factor with a zinc-finger BED domain with structural similarity to the N-terminal DNA-binding domain of the NAC transcription factor (ANAC019) from Arabidopsis. A global gene expression analysis suggests Rph7 mediates the activation and strength of the basal defence response. The isolation of Rph7 highlights the diversification of resistance mechanisms available for engineering disease control in crops.

Date: 2023
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DOI: 10.1038/s41467-023-41021-2

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