Multimodal spatiotemporal transcriptomic resolution of embryonic palate osteogenesis

Jeremie Oliver Piña, Resmi Raju, Daniela M. Roth, Emma Wentworth Winchester, Parna Chattaraj, Fahad Kidwai, Fabio R. Faucz, James Iben, Apratim Mitra, Kiersten Campbell, Gus Fridell, Caroline Esnault, Justin L. Cotney, Ryan K. Dale and Rena N. D’Souza ()
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Jeremie Oliver Piña: National Institutes of Health (NIH)
Resmi Raju: National Institutes of Health (NIH)
Daniela M. Roth: National Institutes of Health (NIH)
Emma Wentworth Winchester: University of Connecticut
Parna Chattaraj: National Institutes of Health (NIH)
Fahad Kidwai: National Institutes of Health (NIH)
Fabio R. Faucz: National Institutes of Health (NIH)
James Iben: National Institutes of Health (NIH)
Apratim Mitra: National Institutes of Health (NIH)
Kiersten Campbell: National Institutes of Health (NIH)
Gus Fridell: National Institutes of Health (NIH)
Caroline Esnault: National Institutes of Health (NIH)
Justin L. Cotney: University of Connecticut
Ryan K. Dale: National Institutes of Health (NIH)
Rena N. D’Souza: National Institutes of Health (NIH)

Nature Communications, 2023, vol. 14, issue 1, 1-15

Abstract: Abstract The terminal differentiation of osteoblasts and subsequent formation of bone marks an important phase in palate development that leads to the separation of the oral and nasal cavities. While the morphogenetic events preceding palatal osteogenesis are well explored, major gaps remain in our understanding of the molecular mechanisms driving the formation of this bony union of the fusing palate. Through bulk, single-nucleus, and spatially resolved RNA-sequencing analyses of the developing secondary palate, we identify a shift in transcriptional programming between embryonic days 14.5 and 15.5 pinpointing the onset of osteogenesis. We define spatially restricted expression patterns of key osteogenic marker genes that are differentially expressed between these developmental timepoints. Finally, we identify genes in the palate highly expressed by palate nasal epithelial cells, also enriched within palatal osteogenic mesenchymal cells. This investigation provides a relevant framework to advance palate-specific diagnostic and therapeutic biomarker discovery.

Date: 2023
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DOI: 10.1038/s41467-023-41349-9

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