Maintenance of pluripotency-like signature in the entire ectoderm leads to neural crest stem cell potential

Ceren Pajanoja, Jenny Hsin, Bradley Olinger, Andrew Schiffmacher, Rita Yazejian, Shaun Abrams, Arvydas Dapkunas, Zarin Zainul, Andrew D. Doyle, Daniel Martin and Laura Kerosuo ()
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Ceren Pajanoja: National Institutes of Health
Jenny Hsin: National Institutes of Health
Bradley Olinger: National Institutes of Health
Andrew Schiffmacher: National Institutes of Health
Rita Yazejian: National Institutes of Health
Shaun Abrams: National Institutes of Health
Arvydas Dapkunas: Department of Biochemistry and Developmental Biology, Faculty of Medicine, University of Helsinki
Zarin Zainul: National Institutes of Health
Andrew D. Doyle: National Institutes of Health
Daniel Martin: National Institutes of Health
Laura Kerosuo: National Institutes of Health

Nature Communications, 2023, vol. 14, issue 1, 1-17

Abstract: Abstract The ability of the pluripotent epiblast to contribute progeny to all three germ layers is thought to be lost after gastrulation. The later-forming neural crest (NC) rises from ectoderm and it remains poorly understood how its exceptionally high stem-cell potential to generate mesodermal- and endodermal-like derivatives is obtained. Here, we monitor transcriptional changes from gastrulation to neurulation using single-cell-Multiplex-Spatial-Transcriptomics (scMST) complemented with RNA-sequencing. We show maintenance of pluripotency-like signature (Nanog, Oct4/PouV, Klf4-positive) in undecided pan-ectodermal stem-cells spanning the entire ectoderm late during neurulation with ectodermal patterning completed only at the end of neurulation when the pluripotency-like signature becomes restricted to NC, challenging our understanding of gastrulation. Furthermore, broad ectodermal pluripotency-like signature is found at multiple axial levels unrelated to the NC lineage the cells later commit to, suggesting a general role in stemness enhancement and proposing a mechanism by which the NC acquires its ability to form derivatives beyond “ectodermal-capacity” in chick and mouse embryos.

Date: 2023
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DOI: 10.1038/s41467-023-41384-6

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