Structural basis of the activation of TRPV5 channels by long-chain acyl-Coenzyme-A

Bo-Hyun Lee, José J. Jesús Pérez, Vera Moiseenkova-Bell () and Tibor Rohacs ()
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Bo-Hyun Lee: Rutgers, New Jersey Medical School
José J. Jesús Pérez: University of Pennsylvania
Vera Moiseenkova-Bell: University of Pennsylvania
Tibor Rohacs: Rutgers, New Jersey Medical School

Nature Communications, 2023, vol. 14, issue 1, 1-14

Abstract: Abstract Long-chain acyl-coenzyme A (LC-CoA) is a crucial metabolic intermediate that plays important cellular regulatory roles, including activation and inhibition of ion channels. The structural basis of ion channel regulation by LC-CoA is not known. Transient receptor potential vanilloid 5 and 6 (TRPV5 and TRPV6) are epithelial calcium-selective ion channels. Here, we demonstrate that LC-CoA activates TRPV5 and TRPV6 in inside-out patches, and both exogenously supplied and endogenously produced LC-CoA can substitute for the natural ligand phosphatidylinositol 4,5-bisphosphate (PI(4,5)P2) in maintaining channel activity in intact cells. Utilizing cryo-electron microscopy, we determined the structure of LC-CoA-bound TRPV5, revealing an open configuration with LC-CoA occupying the same binding site as PI(4,5)P2 in previous studies. This is consistent with our finding that PI(4,5)P2 could not further activate the channels in the presence of LC-CoA. Our data provide molecular insights into ion channel regulation by a metabolic signaling molecule.

Date: 2023
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DOI: 10.1038/s41467-023-41577-z

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