Efficient plant genome engineering using a probiotic sourced CRISPR-Cas9 system

Zhaohui Zhong, Guanqing Liu, Zhongjie Tang, Shuyue Xiang, Liang Yang, Lan Huang, Yao He, Tingting Fan, Shishi Liu, Xuelian Zheng, Tao Zhang, Yiping Qi (), Jian Huang () and Yong Zhang ()
Additional contact information
Zhaohui Zhong: University of Electronic Science and Technology of China
Guanqing Liu: Agricultural College of Yangzhou University
Zhongjie Tang: University of Electronic Science and Technology of China
Shuyue Xiang: University of Electronic Science and Technology of China
Liang Yang: Sichuan Academy of Agricultural Sciences
Lan Huang: University of Electronic Science and Technology of China
Yao He: University of Electronic Science and Technology of China
Tingting Fan: University of Electronic Science and Technology of China
Shishi Liu: University of Electronic Science and Technology of China
Xuelian Zheng: University of Electronic Science and Technology of China
Tao Zhang: Agricultural College of Yangzhou University
Yiping Qi: University of Maryland
Jian Huang: University of Electronic Science and Technology of China
Yong Zhang: University of Electronic Science and Technology of China

Nature Communications, 2023, vol. 14, issue 1, 1-16

Abstract: Abstract Among CRISPR-Cas genome editing systems, Streptococcus pyogenes Cas9 (SpCas9), sourced from a human pathogen, is the most widely used. Here, through in silico data mining, we have established an efficient plant genome engineering system using CRISPR-Cas9 from probiotic Lactobacillus rhamnosus. We have confirmed the predicted 5’-NGAAA-3’ PAM via a bacterial PAM depletion assay and showcased its exceptional editing efficiency in rice, wheat, tomato, and Larix cells, surpassing LbCas12a, SpCas9-NG, and SpRY when targeting the identical sequences. In stable rice lines, LrCas9 facilitates multiplexed gene knockout through coding sequence editing and achieves gene knockdown via targeted promoter deletion, demonstrating high specificity. We have also developed LrCas9-derived cytosine and adenine base editors, expanding base editing capabilities. Finally, by harnessing LrCas9’s A/T-rich PAM targeting preference, we have created efficient CRISPR interference and activation systems in plants. Together, our work establishes CRISPR-LrCas9 as an efficient and user-friendly genome engineering tool for diverse applications in crops and beyond.

Date: 2023
References: View references in EconPapers View complete reference list from CitEc
Citations:

Downloads: (external link)
https://www.nature.com/articles/s41467-023-41802-9 Abstract (text/html)

Related works:
This item may be available elsewhere in EconPapers: Search for items with the same title.

Export reference: BibTeX RIS (EndNote, ProCite, RefMan) HTML/Text

Persistent link: https://EconPapers.repec.org/RePEc:nat:natcom:v:14:y:2023:i:1:d:10.1038_s41467-023-41802-9

Ordering information: This journal article can be ordered from
https://www.nature.com/ncomms/

DOI: 10.1038/s41467-023-41802-9

Access Statistics for this article

Nature Communications is currently edited by Nathalie Le Bot, Enda Bergin and Fiona Gillespie

More articles in Nature Communications from Nature
Bibliographic data for series maintained by Sonal Shukla () and Springer Nature Abstracting and Indexing ().