MYL3 protects chondrocytes from senescence by inhibiting clathrin-mediated endocytosis and activating of Notch signaling

He Cao, Panpan Yang, Jia Liu, Yan Shao, Honghao Li, Pinglin Lai, Hong Wang, Anling Liu, Bin Guo, Yujin Tang, Xiaochun Bai () and Kai Li ()
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He Cao: The Third Affiliated Hospital of Southern Medical University
Panpan Yang: The Third Affiliated Hospital of Southern Medical University
Jia Liu: Affiliated Hospital of Youjiang Medical University for Nationalities
Yan Shao: The Third Affiliated Hospital of Southern Medical University
Honghao Li: The Third Affiliated Hospital of Southern Medical University
Pinglin Lai: The Third Affiliated Hospital of Southern Medical University
Hong Wang: The Third Affiliated Hospital of Southern Medical University
Anling Liu: Southern Medical University
Bin Guo: Southern Medical University
Yujin Tang: Affiliated Hospital of Youjiang Medical University for Nationalities
Xiaochun Bai: The Third Affiliated Hospital of Southern Medical University
Kai Li: The Third Affiliated Hospital of Southern Medical University

Nature Communications, 2023, vol. 14, issue 1, 1-13

Abstract: Abstract As the unique cell type in articular cartilage, chondrocyte senescence is a crucial cellular event contributing to osteoarthritis development. Here we show that clathrin-mediated endocytosis and activation of Notch signaling promotes chondrocyte senescence and osteoarthritis development, which is negatively regulated by myosin light chain 3. Myosin light chain 3 (MYL3) protein levels decline sharply in senescent chondrocytes of cartilages from model mice and osteoarthritis (OA) patients. Conditional deletion of Myl3 in chondrocytes significantly promoted, whereas intra-articular injection of adeno-associated virus overexpressing MYL3 delayed, OA progression in male mice. MYL3 deficiency led to enhanced clathrin-mediated endocytosis by promoting the interaction between myosin VI and clathrin, further inducing the internalization of Notch and resulting in activation of Notch signaling in chondrocytes. Pharmacologic blockade of clathrin-mediated endocytosis-Notch signaling prevented MYL3 loss-induced chondrocyte senescence and alleviated OA progression in male mice. Our results establish a previously unknown mechanism essential for cellular senescence and provide a potential therapeutic direction for OA.

Date: 2023
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DOI: 10.1038/s41467-023-41858-7

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