Fluorescent sensors for imaging of interstitial calcium

Ariel A. Valiente-Gabioud, Inés Garteizgogeascoa Suñer, Agata Idziak, Arne Fabritius, Jérome Basquin, Julie Angibaud, U. Valentin Nägerl, Sumeet Pal Singh and Oliver Griesbeck ()
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Ariel A. Valiente-Gabioud: Tools for Bio-Imaging
Inés Garteizgogeascoa Suñer: Université Libre de Bruxelles (ULB)
Agata Idziak: CNRS - Université de Bordeaux − 146 rue Léo-Saignat
Arne Fabritius: Tools for Bio-Imaging
Jérome Basquin: Max-Planck-Institute for Biochemistry
Julie Angibaud: CNRS - Université de Bordeaux − 146 rue Léo-Saignat
U. Valentin Nägerl: CNRS - Université de Bordeaux − 146 rue Léo-Saignat
Sumeet Pal Singh: Université Libre de Bruxelles (ULB)
Oliver Griesbeck: Tools for Bio-Imaging

Nature Communications, 2023, vol. 14, issue 1, 1-15

Abstract: Abstract Calcium in interstitial fluids is central to systemic physiology and a crucial ion pool for entry into cells through numerous plasma membrane channels. Its study has been limited by the scarcity of methods that allow monitoring in tight inter-cell spaces of living tissues. Here we present high performance ultra-low affinity genetically encoded calcium biosensors named GreenT-ECs. GreenT-ECs combine large fluorescence changes upon calcium binding and binding affinities (Kds) ranging from 0.8 mM to 2.9 mM, making them tuned to calcium concentrations in extracellular organismal fluids. We validated GreenT-ECs in rodent hippocampal neurons and transgenic zebrafish in vivo, where the sensors enabled monitoring homeostatic regulation of tissue interstitial calcium. GreenT-ECs may become useful for recording very large calcium transients and for imaging calcium homeostasis in inter-cell structures in live tissues and organisms.

Date: 2023
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DOI: 10.1038/s41467-023-41928-w

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