Structure of native chromatin fibres revealed by Cryo-ET in situ

Zhen Hou, Frank Nightingale, Yanan Zhu, Craig MacGregor-Chatwin and Peijun Zhang ()
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Zhen Hou: University of Oxford
Frank Nightingale: University of Oxford
Yanan Zhu: University of Oxford
Craig MacGregor-Chatwin: Diamond Light Source, Harwell Science and Innovation Campus
Peijun Zhang: University of Oxford

Nature Communications, 2023, vol. 14, issue 1, 1-7

Abstract: Abstract The structure of chromatin plays pivotal roles in regulating gene transcription, DNA replication and repair, and chromosome segregation. This structure, however, remains elusive. Here, using cryo-FIB and cryo-ET, we delineate the 3D architecture of native chromatin fibres in intact interphase human T-lymphoblasts and determine the in situ structures of nucleosomes in different conformations. These chromatin fibres are not structured as uniform 30 nm one-start or two-start filaments but are composed of relaxed, variable zigzag organizations of nucleosomes connected by straight linker DNA. Nucleosomes with little H1 and linker DNA density are distributed randomly without any spatial preference. This work will inspire future high-resolution investigations on native chromatin structures in situ at both a single-nucleosome level and a population level under many different cellular conditions in health and disease.

Date: 2023
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DOI: 10.1038/s41467-023-42072-1

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