Large-scale recording of neuronal activity in freely-moving mice at cellular resolution

Aniruddha Das, Sarah Holden, Julie Borovicka, Jacob Icardi, Abigail O’Niel, Ariel Chaklai, Davina Patel, Rushik Patel, Stefanie Kaech Petrie, Jacob Raber and Hod Dana ()
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Aniruddha Das: Cleveland Clinic Foundation
Sarah Holden: Oregon Health and Science University
Julie Borovicka: Cleveland Clinic Foundation
Jacob Icardi: Cleveland Clinic Foundation
Abigail O’Niel: Oregon Health and Science University
Ariel Chaklai: Oregon Health and Science University
Davina Patel: Cleveland Clinic Foundation
Rushik Patel: Cleveland Clinic Foundation
Stefanie Kaech Petrie: Oregon Health and Science University
Jacob Raber: Oregon Health and Science University
Hod Dana: Cleveland Clinic Foundation

Nature Communications, 2023, vol. 14, issue 1, 1-12

Abstract: Abstract Current methods for recording large-scale neuronal activity from behaving mice at single-cell resolution require either fixing the mouse head under a microscope or attachment of a recording device to the animal’s skull. Both of these options significantly affect the animal behavior and hence also the recorded brain activity patterns. Here, we introduce a different method to acquire snapshots of single-cell cortical activity maps from freely-moving mice using a calcium sensor called CaMPARI. CaMPARI has a unique property of irreversibly changing its color from green to red inside active neurons when illuminated with 400 nm light. We capitalize on this property to demonstrate cortex-wide activity recording without any head fixation, tethering, or attachment of a miniaturized device to the mouse’s head. Multiple cortical regions were recorded while the mouse was performing a battery of behavioral and cognitive tests. We identified task-dependent activity patterns across motor and somatosensory cortices, with significant differences across sub-regions of the motor cortex and correlations across several activity patterns and task parameters. This CaMPARI-based recording method expands the capabilities of recording neuronal activity from freely-moving and behaving mice under minimally-restrictive experimental conditions and provides large-scale volumetric data that are currently not accessible otherwise.

Date: 2023
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DOI: 10.1038/s41467-023-42083-y

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