Digital data storage on DNA tape using CRISPR base editors

Afsaneh Sadremomtaz, Robert F. Glass, Jorge Eduardo Guerrero, Dennis R. LaJeunesse, Eric A. Josephs () and Reza Zadegan ()
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Afsaneh Sadremomtaz: NC A&T State University
Robert F. Glass: UNC Greensboro
Jorge Eduardo Guerrero: NC A&T State University
Dennis R. LaJeunesse: UNC Greensboro
Eric A. Josephs: UNC Greensboro
Reza Zadegan: NC A&T State University

Nature Communications, 2023, vol. 14, issue 1, 1-10

Abstract: Abstract While the archival digital memory industry approaches its physical limits, the demand is significantly increasing, therefore alternatives emerge. Recent efforts have demonstrated DNA’s enormous potential as a digital storage medium with superior information durability, capacity, and energy consumption. However, the majority of the proposed systems require on-demand de-novo DNA synthesis techniques that produce a large amount of toxic waste and therefore are not industrially scalable and environmentally friendly. Inspired by the architecture of semiconductor memory devices and recent developments in gene editing, we created a molecular digital data storage system called “DNA Mutational Overwriting Storage” (DMOS) that stores information by leveraging combinatorial, addressable, orthogonal, and independent in vitro CRISPR base-editing reactions to write data on a blank pool of greenly synthesized DNA tapes. As a proof of concept, this work illustrates writing and accurately reading of both a bitmap representation of our school’s logo and the title of this study on the DNA tapes.

Date: 2023
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DOI: 10.1038/s41467-023-42223-4

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