Profiling of the Helicobacter pylori redox switch HP1021 regulon using a multi-omics approach

Mateusz Noszka, Agnieszka Strzałka, Jakub Muraszko, Rafał Kolenda, Chen Meng, Christina Ludwig, Kerstin Stingl and Anna Zawilak-Pawlik ()
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Mateusz Noszka: Polish Academy of Sciences
Agnieszka Strzałka: University of Wrocław
Jakub Muraszko: Polish Academy of Sciences
Rafał Kolenda: Wrocław University of Environmental and Life Sciences
Chen Meng: Technical University of Munich (TUM)
Christina Ludwig: Technical University of Munich (TUM)
Kerstin Stingl: German Federal Institute for Risk Assessment
Anna Zawilak-Pawlik: Polish Academy of Sciences

Nature Communications, 2023, vol. 14, issue 1, 1-14

Abstract: Abstract The gastric human pathogen Helicobacter pylori has developed mechanisms to combat stress factors, including reactive oxygen species (ROS). Here, we present a comprehensive study on the redox switch protein HP1021 regulon combining transcriptomic, proteomic and DNA-protein interactions analyses. Our results indicate that HP1021 modulates H. pylori’s response to oxidative stress. HP1021 controls the transcription of 497 genes, including 407 genes related to response to oxidative stress. 79 proteins are differently expressed in the HP1021 deletion mutant. HP1021 controls typical ROS response pathways (katA, rocF) and less canonical ones, particularly DNA uptake and central carbohydrate metabolism. HP1021 is a molecular regulator of competence in H. pylori, as HP1021-dependent repression of the comB DNA uptake genes is relieved under oxidative conditions, increasing natural competence. Furthermore, HP1021 controls glucose consumption by directly regulating the gluP transporter and has an important impact on maintaining the energetic balance in the cell.

Date: 2023
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DOI: 10.1038/s41467-023-42364-6

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