Biosynthesis and engineering of the nonribosomal peptides with a C-terminal putrescine

Hanna Chen, Lin Zhong, Haibo Zhou, Xianping Bai, Tao Sun, Xingyan Wang, Yiming Zhao, Xiaoqi Ji, Qiang Tu, Youming Zhang and Xiaoying Bian ()
Additional contact information
Hanna Chen: Shandong University
Lin Zhong: Chinese Academy of Sciences
Haibo Zhou: Shandong University
Xianping Bai: Shandong University
Tao Sun: Shandong University
Xingyan Wang: Shandong University
Yiming Zhao: Shandong University
Xiaoqi Ji: Shandong University
Qiang Tu: Chinese Academy of Sciences
Youming Zhang: Shandong University
Xiaoying Bian: Shandong University

Nature Communications, 2023, vol. 14, issue 1, 1-17

Abstract: Abstract The broad bioactivities of nonribosomal peptides rely on increasing structural diversity. Genome mining of the Burkholderiales strain Schlegelella brevitalea DSM 7029 leads to the identification of a class of dodecapeptides, glidonins, that feature diverse N-terminal modifications and a uniform putrescine moiety at the C-terminus. The N-terminal diversity originates from the wide substrate selectivity of the initiation module. The C-terminal putrescine moiety is introduced by the unusual termination module 13, the condensation domain directly catalyzes the assembly of putrescine into the peptidyl backbone, and other domains are essential for stabilizing the protein structure. Swapping of this module to another two nonribosomal peptide synthetases leads to the addition of a putrescine to the C-terminus of related nonribosomal peptides, improving their hydrophilicity and bioactivity. This study elucidates the mechanism for putrescine addition and provides further insights to generate diverse and improved nonribosomal peptides by introducing a C-terminal putrescine.

Date: 2023
References: View references in EconPapers View complete reference list from CitEc
Citations:

Downloads: (external link)
https://www.nature.com/articles/s41467-023-42387-z Abstract (text/html)

Related works:
This item may be available elsewhere in EconPapers: Search for items with the same title.

Export reference: BibTeX RIS (EndNote, ProCite, RefMan) HTML/Text

Persistent link: https://EconPapers.repec.org/RePEc:nat:natcom:v:14:y:2023:i:1:d:10.1038_s41467-023-42387-z

Ordering information: This journal article can be ordered from
https://www.nature.com/ncomms/

DOI: 10.1038/s41467-023-42387-z

Access Statistics for this article

Nature Communications is currently edited by Nathalie Le Bot, Enda Bergin and Fiona Gillespie

More articles in Nature Communications from Nature
Bibliographic data for series maintained by Sonal Shukla () and Springer Nature Abstracting and Indexing ().