MSH2 stimulates interfering and inhibits non-interfering crossovers in response to genetic polymorphism

Julia Dluzewska, Wojciech Dziegielewski, Maja Szymanska-Lejman, Monika Gazecka, Ian R. Henderson, James D. Higgins and Piotr A. Ziolkowski ()
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Julia Dluzewska: Adam Mickiewicz University
Wojciech Dziegielewski: Adam Mickiewicz University
Maja Szymanska-Lejman: Adam Mickiewicz University
Monika Gazecka: Adam Mickiewicz University
Ian R. Henderson: University of Cambridge
James D. Higgins: University of Leicester
Piotr A. Ziolkowski: Adam Mickiewicz University

Nature Communications, 2023, vol. 14, issue 1, 1-16

Abstract: Abstract Meiotic crossovers can be formed through the interfering pathway, in which one crossover prevents another from forming nearby, or by an independent non-interfering pathway. In Arabidopsis, local sequence polymorphism between homologs can stimulate interfering crossovers in a MSH2-dependent manner. To understand how MSH2 regulates crossovers formed by the two pathways, we combined Arabidopsis mutants that elevate non-interfering crossovers with msh2 mutants. We demonstrate that MSH2 blocks non-interfering crossovers at polymorphic loci, which is the opposite effect to interfering crossovers. We also observe MSH2-independent crossover inhibition at highly polymorphic sites. We measure recombination along the chromosome arms in lines differing in patterns of heterozygosity and observe a MSH2-dependent crossover increase at the boundaries between heterozygous and homozygous regions. Here, we show that MSH2 is a master regulator of meiotic DSB repair in Arabidopsis, with antagonistic effects on interfering and non-interfering crossovers, which shapes the crossover landscape in relation to interhomolog polymorphism.

Date: 2023
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DOI: 10.1038/s41467-023-42511-z

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