Sialic acid O-acetylation patterns and glycosidic linkage type determination by ion mobility-mass spectrometry

Gaёl M. Vos, Kevin C. Hooijschuur, Zeshi Li, John Fjeldsted, Christian Klein, Robert P. Vries, Javier Sastre Toraño () and Geert-Jan Boons ()
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Gaёl M. Vos: Utrecht University
Kevin C. Hooijschuur: Utrecht University
Zeshi Li: Utrecht University
John Fjeldsted: Agilent Technologies
Christian Klein: Agilent Technologies
Robert P. Vries: Utrecht University
Javier Sastre Toraño: Utrecht University
Geert-Jan Boons: Utrecht University

Nature Communications, 2023, vol. 14, issue 1, 1-13

Abstract: Abstract O-acetylation is a common modification of sialic acids that has been implicated in a multitude of biological and disease processes. A lack of analytical methods that can determine exact structures of sialic acid variants is a hurdle to determine roles of distinct O-acetylated sialosides. Here, we describe a drift tube ion mobility-mass spectrometry approach that can elucidate exact O-acetylation patterns as well as glycosidic linkage types of sialosides isolated from complex biological samples. It is based on the use of a library of synthetic O-acetylated sialosides to establish intrinsic collision cross section (CCS) values of diagnostic fragment ions. The CCS values were used to characterize O-acetylated sialosides from mucins and N-linked glycans from biologicals as well as equine tracheal and nasal tissues. It uncovered contrasting sialic acid linkage types of acetylated and non-acetylated sialic acids and provided a rationale for sialic acid binding preferences of equine H7 influenza A viruses.

Date: 2023
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DOI: 10.1038/s41467-023-42575-x

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