Single amino acid change alters specificity of the multi-allelic wheat stem rust resistance locus SR9

Jianping Zhang, Jayaveeramuthu Nirmala, Shisheng Chen, Matthias Jost, Burkhard Steuernagel, Mirka Karafiatova, Tim Hewitt, Hongna Li, Erena Edae, Keshav Sharma, Sami Hoxha, Dhara Bhatt, Rea Antoniou-Kourounioti, Peter Dodds, Brande B. H. Wulff, Jaroslav Dolezel, Michael Ayliffe, Colin Hiebert, Robert McIntosh, Jorge Dubcovsky, Peng Zhang (), Matthew N. Rouse () and Evans Lagudah ()
Additional contact information
Jianping Zhang: CSIRO Agriculture & Food
Jayaveeramuthu Nirmala: University of Minnesota
Shisheng Chen: Peking University Institute of Advanced Agricultural Sciences, Shandong Laboratory of Advanced Agricultural Sciences at Weifang
Matthias Jost: CSIRO Agriculture & Food
Burkhard Steuernagel: John Innes Centre, Norwich Research Park
Mirka Karafiatova: Centre of the Region Haná for Biotechnological and Agricultural Research
Tim Hewitt: CSIRO Agriculture & Food
Hongna Li: Peking University Institute of Advanced Agricultural Sciences, Shandong Laboratory of Advanced Agricultural Sciences at Weifang
Erena Edae: University of Minnesota
Keshav Sharma: Cereal Disease Laboratory
Sami Hoxha: University of Sydney
Dhara Bhatt: CSIRO Agriculture & Food
Rea Antoniou-Kourounioti: University of Glasgow
Peter Dodds: CSIRO Agriculture & Food
Brande B. H. Wulff: King Abdullah University of Science and Technology (KAUST)
Jaroslav Dolezel: Centre of the Region Haná for Biotechnological and Agricultural Research
Michael Ayliffe: CSIRO Agriculture & Food
Colin Hiebert: Agriculture and Agri-Food Canada, Morden Research and Development Centre
Robert McIntosh: University of Sydney
Jorge Dubcovsky: University of California
Peng Zhang: University of Sydney
Matthew N. Rouse: University of Minnesota
Evans Lagudah: CSIRO Agriculture & Food

Nature Communications, 2023, vol. 14, issue 1, 1-12

Abstract: Abstract Most rust resistance genes thus far isolated from wheat have a very limited number of functional alleles. Here, we report the isolation of most of the alleles at wheat stem rust resistance gene locus SR9. The seven previously reported resistance alleles (Sr9a, Sr9b, Sr9d, Sr9e, Sr9f, Sr9g, and Sr9h) are characterised using a synergistic strategy. Loss-of-function mutants and/or transgenic complementation are used to confirm Sr9b, two haplotypes of Sr9e (Sr9e_h1 and Sr9e_h2), Sr9g, and Sr9h. Each allele encodes a highly related nucleotide-binding site leucine-rich repeat (NB-LRR) type immune receptor, containing an unusual long LRR domain, that confers resistance to a unique spectrum of isolates of the wheat stem rust pathogen. The only SR9 protein effective against stem rust pathogen race TTKSK (Ug99), SR9H, differs from SR9B by a single amino acid. SR9B and SR9G resistance proteins are also distinguished by only a single amino acid. The SR9 allelic series found in the B subgenome are orthologs of wheat stem rust resistance gene Sr21 located in the A subgenome with around 85% identity in protein sequences. Together, our results show that functional diversification of allelic variants at the SR9 locus involves single and multiple amino acid changes that recognize isolates of wheat stem rust.

Date: 2023
References: View references in EconPapers View complete reference list from CitEc
Citations:

Downloads: (external link)
https://www.nature.com/articles/s41467-023-42747-9 Abstract (text/html)

Related works:
This item may be available elsewhere in EconPapers: Search for items with the same title.

Export reference: BibTeX RIS (EndNote, ProCite, RefMan) HTML/Text

Persistent link: https://EconPapers.repec.org/RePEc:nat:natcom:v:14:y:2023:i:1:d:10.1038_s41467-023-42747-9

Ordering information: This journal article can be ordered from
https://www.nature.com/ncomms/

DOI: 10.1038/s41467-023-42747-9

Access Statistics for this article

Nature Communications is currently edited by Nathalie Le Bot, Enda Bergin and Fiona Gillespie

More articles in Nature Communications from Nature
Bibliographic data for series maintained by Sonal Shukla () and Springer Nature Abstracting and Indexing ().