RNA recognition by Npl3p reveals U2 snRNA-binding compatible with a chaperone role during splicing

Ahmed Moursy, Antoine Cléry (), Stefan Gerhardy, Katharina M. Betz, Sanjana Rao, Jarosław Mazur, Sébastien Campagne, Irene Beusch, Malgorzata M. Duszczyk, Mark D. Robinson, Vikram Govind Panse () and Frédéric H.-T. Allain ()
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Ahmed Moursy: Institute of Biochemistry
Antoine Cléry: Institute of Biochemistry
Stefan Gerhardy: Institute of Biochemistry
Katharina M. Betz: University of Zurich
Sanjana Rao: University of Zurich
Jarosław Mazur: University of Zurich
Sébastien Campagne: Institute of Biochemistry
Irene Beusch: Institute of Biochemistry
Malgorzata M. Duszczyk: Institute of Biochemistry
Mark D. Robinson: University of Zurich
Vikram Govind Panse: University of Zurich
Frédéric H.-T. Allain: Institute of Biochemistry

Nature Communications, 2023, vol. 14, issue 1, 1-15

Abstract: Abstract The conserved SR-like protein Npl3 promotes splicing of diverse pre-mRNAs. However, the RNA sequence(s) recognized by the RNA Recognition Motifs (RRM1 & RRM2) of Npl3 during the splicing reaction remain elusive. Here, we developed a split-iCRAC approach in yeast to uncover the consensus sequence bound to each RRM. High-resolution NMR structures show that RRM2 recognizes a 5´-GNGG-3´ motif leading to an unusual mille-feuille topology. These structures also reveal how RRM1 preferentially interacts with a CC-dinucleotide upstream of this motif, and how the inter-RRM linker and the region C-terminal to RRM2 contribute to cooperative RNA-binding. Structure-guided functional studies show that Npl3 genetically interacts with U2 snRNP specific factors and we provide evidence that Npl3 melts U2 snRNA stem-loop I, a prerequisite for U2/U6 duplex formation within the catalytic center of the Bact spliceosomal complex. Thus, our findings suggest an unanticipated RNA chaperoning role for Npl3 during spliceosome active site formation.

Date: 2023
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DOI: 10.1038/s41467-023-42962-4

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