Imaging cellular forces with photonic crystals

Qiwei Li, Zaozao Chen, Ying Zhang, Shuang Ding, Haibo Ding, Luping Wang, Zhuoying Xie, Yifu Fu, Mengxiao Wei, Shengnan Liu, Jialun Chen, Xuan Wang and Zhongze Gu ()
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Qiwei Li: Southeast University
Zaozao Chen: Southeast University
Ying Zhang: Southeast University
Shuang Ding: Southeast University
Haibo Ding: Southeast University
Luping Wang: Southeast University
Zhuoying Xie: Southeast University
Yifu Fu: Southeast University
Mengxiao Wei: Southeast University
Shengnan Liu: Southeast University
Jialun Chen: Southeast University
Xuan Wang: Southeast University
Zhongze Gu: Southeast University

Nature Communications, 2023, vol. 14, issue 1, 1-14

Abstract: Abstract Current techniques for visualizing and quantifying cellular forces have limitations in live cell imaging, throughput, and multi-scale analysis, which impede progress in cell force research and its practical applications. We developed a photonic crystal cellular force microscopy (PCCFM) to image vertical cell forces over a wide field of view (1.3 mm ⨯ 1.0 mm, a 10 ⨯ objective image) at high speed (about 20 frames per second) without references. The photonic crystal hydrogel substrate (PCS) converts micro-nano deformations into perceivable color changes, enabling in situ visualization and quantification of tiny vertical cell forces with high throughput. It enabled long-term, cross-scale monitoring from subcellular focal adhesions to tissue-level cell sheets and aggregates.

Date: 2023
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DOI: 10.1038/s41467-023-43090-9

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