Site-selected in situ polymerization for living cell surface engineering

Yihong Zhong, Lijia Xu, Chen Yang, Le Xu, Guyu Wang, Yuna Guo, Songtao Cheng, Xiao Tian, Changjiang Wang, Ran Xie, Xiaojian Wang (), Lin Ding () and Huangxian Ju
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Yihong Zhong: Nanjing University
Lijia Xu: Nanjing University
Chen Yang: Nanjing University
Le Xu: Nanjing Tech University
Guyu Wang: Nanjing University
Yuna Guo: Nanjing University
Songtao Cheng: Nanjing University
Xiao Tian: Nanjing University
Changjiang Wang: Nanjing University
Ran Xie: Nanjing University
Xiaojian Wang: Nanjing Tech University
Lin Ding: Nanjing University
Huangxian Ju: Nanjing University

Nature Communications, 2023, vol. 14, issue 1, 1-17

Abstract: Abstract The construction of polymer-based mimicry on cell surface to manipulate cell behaviors and functions offers promising prospects in the field of biotechnology and cell therapy. However, precise control of polymer grafting sites is essential to successful implementation of biomimicry and functional modulation, which has been overlooked by most current research. Herein, we report a biological site-selected, in situ controlled radical polymerization platform for living cell surface engineering. The method utilizes metabolic labeling techniques to confine the growth sites of polymers and designs a Fenton-RAFT polymerization technique with cytocompatibility. Polymers grown at different sites (glycans, proteins, lipids) have different membrane retention time and exhibit differential effects on the recognition behaviors of cellular glycans. Of particular importance is the achievement of in situ copolymerization of glycomonomers on the outermost natural glycan sites of cell membrane, building a biomimetic glycocalyx with distinct recognition properties.

Date: 2023
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DOI: 10.1038/s41467-023-43161-x

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