Loss-of-function of an α-SNAP gene confers resistance to soybean cyst nematode

Mariola Usovsky, Vinavi A. Gamage, Clinton G. Meinhardt, Nicholas Dietz, Marissa Triller, Pawan Basnet, Jason D. Gillman, Kristin D. Bilyeu, Qijian Song, Bishnu Dhital, Alice Nguyen, Melissa G. Mitchum () and Andrew M. Scaboo ()
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Mariola Usovsky: University of Missouri
Vinavi A. Gamage: University of Georgia
Clinton G. Meinhardt: University of Missouri
Nicholas Dietz: University of Missouri
Marissa Triller: University of Missouri
Pawan Basnet: University of Missouri
Jason D. Gillman: University of Missouri
Kristin D. Bilyeu: University of Missouri
Qijian Song: Beltsville Agricultural Research Center
Bishnu Dhital: University of Missouri
Alice Nguyen: University of Missouri
Melissa G. Mitchum: University of Georgia
Andrew M. Scaboo: University of Missouri

Nature Communications, 2023, vol. 14, issue 1, 1-14

Abstract: Abstract Plant-parasitic nematodes are one of the most economically impactful pests in agriculture resulting in billions of dollars in realized annual losses worldwide. Soybean cyst nematode (SCN) is the number one biotic constraint on soybean production making it a priority for the discovery, validation and functional characterization of native plant resistance genes and genetic modes of action that can be deployed to improve soybean yield across the globe. Here, we present the discovery and functional characterization of a soybean resistance gene, GmSNAP02. We use unique bi-parental populations to fine-map the precise genomic location, and a combination of whole genome resequencing and gene fragment PCR amplifications to identify and confirm causal haplotypes. Lastly, we validate our candidate gene using CRISPR-Cas9 genome editing and observe a gain of resistance in edited plants. This demonstrates that the GmSNAP02 gene confers a unique mode of resistance to SCN through loss-of-function mutations that implicate GmSNAP02 as a nematode virulence target. We highlight the immediate impact of utilizing GmSNAP02 as a genome-editing-amenable target to diversify nematode resistance in commercially available cultivars.

Date: 2023
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DOI: 10.1038/s41467-023-43295-y

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