Induced formation of primordial germ cells from zebrafish blastomeres by germplasm factors

Xiaosi Wang, Junwen Zhu, Houpeng Wang, Wenqi Deng, Shengbo Jiao, Yaqing Wang, Mudan He, Fenghua Zhang, Tao Liu, Yongkang Hao, Ding Ye and Yonghua Sun ()
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Xiaosi Wang: State Key Laboratory of Freshwater Ecology and Biotechnology, Key Laboratory of Breeding Biotechnology and Sustainable Aquaculture, Institute of Hydrobiology, Innovation Academy for Seed Design, Chinese Academy of Sciences
Junwen Zhu: State Key Laboratory of Freshwater Ecology and Biotechnology, Key Laboratory of Breeding Biotechnology and Sustainable Aquaculture, Institute of Hydrobiology, Innovation Academy for Seed Design, Chinese Academy of Sciences
Houpeng Wang: State Key Laboratory of Freshwater Ecology and Biotechnology, Key Laboratory of Breeding Biotechnology and Sustainable Aquaculture, Institute of Hydrobiology, Innovation Academy for Seed Design, Chinese Academy of Sciences
Wenqi Deng: State Key Laboratory of Freshwater Ecology and Biotechnology, Key Laboratory of Breeding Biotechnology and Sustainable Aquaculture, Institute of Hydrobiology, Innovation Academy for Seed Design, Chinese Academy of Sciences
Shengbo Jiao: State Key Laboratory of Freshwater Ecology and Biotechnology, Key Laboratory of Breeding Biotechnology and Sustainable Aquaculture, Institute of Hydrobiology, Innovation Academy for Seed Design, Chinese Academy of Sciences
Yaqing Wang: State Key Laboratory of Freshwater Ecology and Biotechnology, Key Laboratory of Breeding Biotechnology and Sustainable Aquaculture, Institute of Hydrobiology, Innovation Academy for Seed Design, Chinese Academy of Sciences
Mudan He: State Key Laboratory of Freshwater Ecology and Biotechnology, Key Laboratory of Breeding Biotechnology and Sustainable Aquaculture, Institute of Hydrobiology, Innovation Academy for Seed Design, Chinese Academy of Sciences
Fenghua Zhang: State Key Laboratory of Freshwater Ecology and Biotechnology, Key Laboratory of Breeding Biotechnology and Sustainable Aquaculture, Institute of Hydrobiology, Innovation Academy for Seed Design, Chinese Academy of Sciences
Tao Liu: State Key Laboratory of Freshwater Ecology and Biotechnology, Key Laboratory of Breeding Biotechnology and Sustainable Aquaculture, Institute of Hydrobiology, Innovation Academy for Seed Design, Chinese Academy of Sciences
Yongkang Hao: State Key Laboratory of Freshwater Ecology and Biotechnology, Key Laboratory of Breeding Biotechnology and Sustainable Aquaculture, Institute of Hydrobiology, Innovation Academy for Seed Design, Chinese Academy of Sciences
Ding Ye: State Key Laboratory of Freshwater Ecology and Biotechnology, Key Laboratory of Breeding Biotechnology and Sustainable Aquaculture, Institute of Hydrobiology, Innovation Academy for Seed Design, Chinese Academy of Sciences
Yonghua Sun: State Key Laboratory of Freshwater Ecology and Biotechnology, Key Laboratory of Breeding Biotechnology and Sustainable Aquaculture, Institute of Hydrobiology, Innovation Academy for Seed Design, Chinese Academy of Sciences

Nature Communications, 2023, vol. 14, issue 1, 1-11

Abstract: Abstract The combination of genome editing and primordial germ cell (PGC) transplantation has enormous significance in the study of developmental biology and genetic breeding, despite its low efficiency due to limited number of donor PGCs. Here, we employ a combination of germplasm factors to convert blastoderm cells into induced PGCs (iPGCs) in zebrafish and obtain functional gametes either through iPGC transplantation or via the single blastomere overexpression of germplasm factors. Zebrafish-derived germplasm factors convert blastula cells of Gobiocypris rarus into iPGCs, and Gobiocypris rarus spermatozoa can be produced by iPGC-transplanted zebrafish. Moreover, the combination of genome knock-in and iPGC transplantation perfectly resolves the contradiction between high knock-in efficiency and early lethality during embryonic stages and greatly improves the efficiency of genome knock-in. Together, we present an efficient method for generating PGCs in a teleost, a technique that will have a strong impact in basic research and aquaculture.

Date: 2023
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DOI: 10.1038/s41467-023-43587-3

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