Discovery and remodeling of Vibrio natriegens as a microbial platform for efficient formic acid biorefinery

Jinzhong Tian (), Wangshuying Deng, Ziwen Zhang, Jiaqi Xu, Guiling Yang, Guoping Zhao, Sheng Yang, Weihong Jiang () and Yang Gu ()
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Jinzhong Tian: Chinese Academy of Sciences
Wangshuying Deng: Chinese Academy of Sciences
Ziwen Zhang: Chinese Academy of Sciences
Jiaqi Xu: ZJU-Hangzhou Global Scientific and Technological Innovation Center
Guiling Yang: Xianghu Laboratory
Guoping Zhao: Chinese Academy of Sciences
Sheng Yang: Chinese Academy of Sciences
Weihong Jiang: Chinese Academy of Sciences
Yang Gu: Chinese Academy of Sciences

Nature Communications, 2023, vol. 14, issue 1, 1-12

Abstract: Abstract Formic acid (FA) has emerged as a promising one-carbon feedstock for biorefinery. However, developing efficient microbial hosts for economically competitive FA utilization remains a grand challenge. Here, we discover that the bacterium Vibrio natriegens has exceptional FA tolerance and metabolic capacity natively. This bacterium is remodeled by rewiring the serine cycle and the TCA cycle, resulting in a non-native closed loop (S-TCA) which as a powerful metabolic sink, in combination with laboratory evolution, enables rapid emergence of synthetic strains with significantly improved FA-utilizing ability. Further introduction of a foreign indigoidine-forming pathway into the synthetic V. natriegens strain leads to the production of 29.0 g · L−1 indigoidine and consumption of 165.3 g · L−1 formate within 72 h, achieving a formate consumption rate of 2.3 g · L−1 · h−1. This work provides an important microbial chassis as well as design rules to develop industrially viable microorganisms for FA biorefinery.

Date: 2023
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DOI: 10.1038/s41467-023-43631-2

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