Nearly complete structure of bacteriophage DT57C reveals architecture of head-to-tail interface and lateral tail fibers

Rafael Ayala, Andrey V. Moiseenko, Ting-Hua Chen, Eugene E. Kulikov, Alla K. Golomidova, Philipp S. Orekhov, Maya A. Street, Olga S. Sokolova (), Andrey V. Letarov () and Matthias Wolf ()
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Rafael Ayala: Okinawa Institute of Science and Technology Graduate University
Andrey V. Moiseenko: Lomonosov Moscow State University
Ting-Hua Chen: Okinawa Institute of Science and Technology Graduate University
Eugene E. Kulikov: Lomonosov Moscow State University
Alla K. Golomidova: Research Center of Biotechnology of the Russian Academy of Sciences
Philipp S. Orekhov: Shenzhen MSU-BIT University
Maya A. Street: Okinawa Institute of Science and Technology Graduate University
Olga S. Sokolova: Lomonosov Moscow State University
Andrey V. Letarov: Lomonosov Moscow State University
Matthias Wolf: Okinawa Institute of Science and Technology Graduate University

Nature Communications, 2023, vol. 14, issue 1, 1-14

Abstract: Abstract The T5 family of viruses are tailed bacteriophages characterized by a long non-contractile tail. The bacteriophage DT57C is closely related to the paradigmal T5 phage, though it recognizes a different receptor (BtuB) and features highly divergent lateral tail fibers (LTF). Considerable portions of T5-like phages remain structurally uncharacterized. Here, we present the structure of DT57C determined by cryo-EM, and an atomic model of the virus, which was further explored using all-atom molecular dynamics simulations. The structure revealed a unique way of LTF attachment assisted by a dodecameric collar protein LtfC, and an unusual composition of the phage neck constructed of three protein rings. The tape measure protein (TMP) is organized within the tail tube in a three-stranded parallel α-helical coiled coil which makes direct contact with the genomic DNA. The presence of the C-terminal fragment of the TMP that remains within the tail tip suggests that the tail tip complex returns to its original state after DNA ejection. Our results provide a complete atomic structure of a T5-like phage, provide insights into the process of DNA ejection as well as a structural basis for the design of engineered phages and future mechanistic studies.

Date: 2023
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Persistent link: https://EconPapers.repec.org/RePEc:nat:natcom:v:14:y:2023:i:1:d:10.1038_s41467-023-43824-9

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DOI: 10.1038/s41467-023-43824-9

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